Demographic effects on the use of genetic options for the control of mosquitofish, Gambusia holbrooki.

This study tests the sensitivity of genetically based pest control options based on sex ratio distortion to intra- and intersexual aggressive interactions that affect male and female survival and fitness. Data on these interactions and their impacts were gathered for the mosquitofish Gambusia holbrooki (Poeciliidae), a promiscuous species with a strongly male-biased operational sex ratio and well-documented male harassment of females. The experimental design consisted of an orthogonal combination of two population densities and three sex ratios, ranging from strongly male-biased to strongly female-biased, and long-term observations of laboratory populations.

Effects of lifetime chemical inhibition of aromatase on the sexual differentiation, sperm characteristics and fertility of medaka (Oryzias latipes) and zebrafish (Danio rerio).

The deleterious effects of tributyltin (TBT) on spermiation in fish have been attributed to its role in inhibiting aromatisation of androgens to estrogens, and the critical role of the latter in sperm development. We test this hypothesis by examining sperm parameters, fertilisation and hatching success in males of two fish species exposed throughout life to doses of Fadrozole, a non-steroidal aromatase inhibitor (AI), provided in their diets. AI-treatment caused 100% male development in zebrafish, but only partial masculinisation in medaka, in both cases supporting previous studies and suggesting different roles of estrogen in sexual differentiation in the two species.