Publications by authors named "Miles A Taylor"
Article Synopsis
- Green fluorescent protein (GFP) has transformed bioimaging, but outside its natural environment, the GFP chromophore (HBDI) lacks fluorescence, prompting research into its behavior in solution.
- This study uses femtosecond stimulated Raman spectroscopy (FSRS) and transient absorption (TA) to analyze how energy dissipates during the HBDI chromophore's transition through a conical intersection, revealing key vibrational couplings.
- The findings highlight a delayed coupling onset related to the charge-separated state prior to reaching the TICT state, offering insights into photochemical reactions and laying groundwork for designing efficient molecular systems like nanomachines and fluorescent markers.
Tracking vibrational motions during a photochemical or photophysical process has gained momentum, due to its sensitivity to the progression of reaction and change of environment. In this work, we implemented an advanced ultrafast vibrational technique, femtosecond-stimulated Raman spectroscopy (FSRS), to monitor the excited state structural evolution of an engineered green fluorescent protein (GFP) single-site mutant S205V. This mutation alters the original excited state proton transfer (ESPT) chain.
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