Insights into Early Ontogenesis of : RNA Extraction, Housekeeping Gene Validation and Transcriptional Expression of Important Primordial Germ Cell and Sex-Determination Genes.

The challenge in extracting high-quality RNA impedes the investigation of the transcriptome of developing salmonid embryos. Furthermore, the mRNA expression pattern of important PGC and SD genes during the initial embryonic development of is yet to be studied. So, in the present study, we aimed to isolate high-quality RNA from eggs and developing embryos to check , , , , , , , and expression by qPCR.