Toxicity of different-sized cobalt ferrite (CoFeO) nanoparticles to Oncorhynchus mykiss at early development stages.

As innovative and versatile agents with potential applications in a wide range of fields including medicine, electronics, wastewater treatment, cosmetics, and energy storage devices, magnetic nanoparticles (NPs) are significant attention. However, our knowledge of the harmful effects of different-sized NPs, particularly of their effects on aquatic animals, is limited. In this study, we evaluated the impact of different-sized (sub-2, 5, and 15 nm) cobalt ferrite (CoFeO) NPs on the biological parameters of rainbow trout (Oncorhynchus mykiss) embryos and larvae.

Effects of handling and surgical implant on juvenile wild trout: Physiological and behavioral impact.

Fish involved in telemetry studies are usually handled, anaesthetized, and subjected to internal tag implantation, all of which have the potential to disrupt the fish's physiology, migratory patterns, food-seeking behavior, growth, and survival. As fish tagging is a widespread standard method in aquaculture, it is crucial to gain a better understanding of the short- and long-term effects on various aspects of fish welfare. The experimental outcomes of each phase of the surgical process and how it affects the fish's ability to recover during the post-tagging period, particularly in small salmonids, are poorly understood.

Diversity of fungus-like stramenopilous organisms (Oomycota) in Lithuanian freshwater aquaculture: Morphological and molecular analysis, risk to fish health.

The present work is the first comprehensive study of fungus-like stramenopilous organisms (Oomycota) diversity in Lithuanian fish farms aimed at proper identification of saprolegniasis pathogens, which is important for water quality control, monitoring infection levels and choosing more effective treatments for this disease in aquaculture. Pathogenic to fish, Saprolegnia and other potentially pathogenic water moulds were isolated from adult fish, their eggs, fry and from water samples. All detected isolates were examined morphologically and confirmed by sequence-based molecular methods.

A multiscale study of the effects of a diet containing CdSe/ZnS-COOH quantum dots on Salmo trutta fario L.: Potential feed-related nanotoxicity.

Quantum dots (QDs) receive widespread attention in industrial and biomedical fields, but the risks posed by the use of nanoparticles to aquatic organisms and the associated toxicological effects are still not well understood. In this study, effects of the 7-day dietary exposure of Salmo trutta fario L. juveniles to CdSe/ZnS-COOH QDs were evaluated at molecular, cellular, physiological and whole-organism levels.

A comparative analysis of multi-biomarker responses to environmental stress: Evaluating differences in landfill leachate and pathogenic oomycete effects between wild and captive Salmo trutta.

Phenotypic plasticity is one of the major means by which organisms can manage with environmental factor changes. Captivity-related stress and artificial rearing settings have been shown to dramatically alter fish response plasticity in terms of physiology, behavior, and health, potentially reducing overall fitness and fish survival. Understanding the variations in plasticity between captive-bred (kept in a homogenous environment) and wild fish populations in response to varied environmental pressures is becoming increasingly important, particularly in risk assessment research.

Biomarker responses in mussels (Mytilus trossulus) from the Baltic Sea exposed to water-accommodated fraction of crude oil and a dispersant at different salinities.

Oil spills pose significant environmental risks, particularly in cold seas. In the Baltic Sea, the low salinity (from 0 to 2 up to 18) affects the behaviour of the spilled oil as well as the efficiency and ecological impacts of oil spill response methods such as mechanical collection and the use of dispersants. In the present study, mussels (Mytilus trossulus) were exposed under winter conditions (5 °C) to the water-accommodated fraction (WAF) of Naphthenic North Atlantic crude oil prepared by mechanical dispersion or to the chemically enhanced fraction (CEWAF) obtained using the dispersant Finasol OSR 51 at salinities of 5.

The impact of co-treatment with graphene oxide and metal mixture on Salmo trutta at early development stages: The sorption capacity and potential toxicity.

Graphene oxide (GO) are novel nanomaterials with a wide range of applications due to their high absorption capacity. This study was undertaken with a view to assess the bioaccumulation and acute toxicity of GO used in combination with the heavy metal mixture (Cr, Cu, Ni and Zn) to fish embryos and larvae. For this purpose, Salmo trutta embryos and larvae were subjected to the 4-day long treatment with three different concentrations of GO, the metal mixture, which was prepared of four metals at the concentrations corresponding to the maximum-permissible-concentrations for EU inland waters (Cr-0.

Technical suitability and reliability of an in vivo and non-invasive biosensor-type glucose assessment as a potential biomarker for multiple stressors in fishes: an evaluation on Salmonids.

Regardless of the wide use of glucose measurements in stress evaluation, there are some inconsistencies in its acceptance as a stress marker. To meet the challenge and test the reliability/suitability of glucose measurement in practice, we simulated different environmental/anthropogenic exposure scenarios in this study. We aimed to provoke stress in fish followed by a 2-week stress recovery period and under the cumulative effect of leachate fish exposed to pathogenic oomycetes (Saprolegnia parasitica) to represent a possible infection in fish.

Effects of different types of primary microplastics on early life stages of rainbow trout (Oncorhynchus mykiss).

Plastic pollution is recognized as serious threat to aquatic organisms. The aim of this research was to determine the effect of environmentally realistic concentrations of various microplastics (MPs) on survival, growth, development and induction of endocrine, geno- and cytotoxic responses in the early life stages of rainbow trout Oncorhynchus mykiss. Fish were exposed for 69-days, from embryos at eyed-stage to mobile yolk-sac larvae, to pre-production pellets (3000 μm; polystyrene - PS and polyethylene terephthalate - PET).

Swimming behaviour in two ecologically similar three-spined (Gasterosteus aculeatus L.) and nine-spined sticklebacks (Pungitius pungitius L.): a comparative approach for modelling the toxicity of metal mixtures.

Sticklebacks (Gasterosteiformes) are increasingly used in ecological and evolutionary research and have become well established as role model species for biologists. However, ecotoxicology studies concerning behavioural effects in sticklebacks regarding stress responses, mainly induced by chemical mixtures, have hardly been addressed. For this purpose, we investigated the swimming behaviour (including mortality rate based on 96-h LC values) of two ecologically similar three-spined (Gasterosteus aculeatus) and nine-spined sticklebacks (Pungitius pungitius) to short-term (up to 24 h) metal mixture (MIX) exposure.

Biomarker responses in perch (Perca fluviatilis) under multiple stress: Parasite co-infection and multicomponent metal mixture exposure.

Parasitic infections may cause damage to the host immune system (i.e. fish), thereby endangering its health and weakening its responses to other types of stressors.

Cytogenetic damage in native Baltic Sea fish species: environmental risks associated with chemical munition dumping in the Gotland Basin of the Baltic Sea.

This study represents the first attempt to assess genotoxicity and cytotoxicity effects in herring (Clupea harengus membras), flounder (Platichthys flesus), and cod (Gadus morhua callarias) caught at 47 study stations, located close to chemical munition dumpsites in the Gotland Basin, the Baltic Sea. Herring sampled from stations located in the center of chemical munition dumpsites exhibited the highest levels of micronuclei (MN) and total genotoxicity (ΣGentox), which is defined as the sum of frequencies of such nuclear abnormalities as micronuclei, nuclear buds, nuclear buds on the filament, and bi-nucleated erythrocytes with nucleoplasmic bridges. Exceptionally high and high ΣGentox risks were determined for flounder (89.

Biological effects of multimetal (Ni, Cd, Pb, Cu, Cr, Zn) mixture in rainbow trout Oncorhynchus mykiss: Laboratory exposure and recovery study.

The present study tested the biological consequences of exposure to a multimetal mixture as a multiple chemical stressor on Oncorhynchus mykiss at molecular, cellular, physiological and whole-organism levels and on biomarker responses of this fish during the depuration period. To represent environmentally relevant multiple chemical stressors, in our study, we used the mixture of Zn, Cu, Ni, Cr, Pb and Cd at the concentrations corresponding to Maximum-Permissible-Concentrations (MPCs) acceptable for the EU inland waters. This study was undertaken with a view to elucidate if changes in the MPC of the test mixture components (Ni, Pb, Cd) could cause significantly different biomarker responses in O.

Acclimation effect on fish behavioural characteristics: determination of appropriate acclimation period for different species.

In the present study, the authors investigated the effect of acclimation duration (up to 4 h) on behavioural characteristics of taxonomically and functionally different fish species, i.e., the migratory rheophilic salmonids rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar), and the non-migratory eurytopic European perch (Perca fluviatilis) and three-spined stickleback (Gasterosteus aculeatus).

Effects of chronic exposure to microplastics of different polymer types on early life stages of sea trout Salmo trutta.

The aim of the present study was to determine the effect of a long-term (113 days) exposure to microplastics on the development and induction of endocrine, geno- and cytotoxic responses in early life stages of sea trout Salmo trutta. Microplastic particles (3000 μm) of three most commonly mass-produced polymers (polystyrene - PS, polyethylene terephthalate - PET and polyethylene - PE) were applied in environmentally realistic concentrations (0.1% of sediment dry weight) in a laboratory experiment imitating the natural environment, typical for sea trout spawning grounds.

Biological effects of dumped chemical weapons in the Baltic Sea: A multi-biomarker study using caged mussels at the Bornholm main dumping site.

After World War II, thousands of tons of highly toxic chemical warfare agents (CWA) were deposited in the Baltic Sea, the main dumping site locating in the Bornholm Basin. In the present study, Baltic mussels (Mytilus trossulus) were transplanted in the area in cages at two hotspot sites and a reference site at the depths of 35 and 65 m for 2.5 months to study bioaccumulation and biological effects of CWA possibly leaking from the corroding warfare materials.

Biomarker responses and accumulation of polycyclic aromatic hydrocarbons in Mytilus trossulus and Gammarus oceanicus during exposure to crude oil.

In the brackish water Baltic Sea, oil pollution is an ever-present and significant environmental threat mainly due to the continuously increasing volume of oil transport in the area. In this study, effects of exposure to crude oil on two common Baltic Sea species, the mussel Mytilus trossulus and the amphipod Gammarus oceanicus, were investigated. The species were exposed for various time periods (M.

Response patterns of biomarkers in omnivorous and carnivorous fish species exposed to multicomponent metal (Cd, Cr, Cu, Ni, Pb and Zn) mixture. Part III.

Toxicity to fish of multicomponent metal mixtures at maximum-permissible-concentrations (MPC: Cd-0.005, Cr-0.01, Cu-0.

Juvenile fish responses to sublethal leachate concentrations: comparison of sensitivity of different behavioral endpoints.

The aim of the present study was to investigate changes in the locomotor activity of rainbow trout (Oncorhynchus mykiss) juveniles under the impact (2 h) of landfill leachate (as a multicomponent mixture) based on different endpoints such as average, maximum and angular velocities, movement duration, body mobility, and blood glucose level. Fish were exposed to five different sublethal leachate concentrations (0, 0.0625, 0.

Toxicological Potential of Cadmium Impact on Rainbow Trout (Oncorhynchus mykiss) in Early Development.

Cadmium (Cd) is a toxic element widely distributed in the aquatic environment and producing a wide variety of harmful effects. In this study, the acute toxicity (96 h LC50) of Cd to rainbow trout Oncorhynchus mykiss embryos and larvae was determined. The obtained results showed that hatched larvae were the most sensitive to Cd exposure.

Rapidness- and Sensitivity-Based Comparison of Behavioral and Respiratory Responses of European Perch and Rainbow Trout to Metal Mixture Exposure.

The aim of the study was to investigate acute (96-h LC50) toxicity of the complex metal (Zn, Pb, Cd, Cu, Ni and Cr) mixture (MIX) to European perch (Perca fluviatilis) and rainbow trout (Oncorhynchus mykiss), and to examine differences between locomotor and respiratory endpoints based on rapidness and sensitivity of fish responses to short-term (2 h) metal mixture exposure. MIX was prepared taking into consideration the maximum-permissible-concentrations (MPC) set for these metals in EU inland waters (Directive 2008/105/EC). The studied behavioral and respiratory responses of O.

Induction of nuclear abnormalities in herring (Clupea harengus membras), flounder (Platichthys flesus), and Atlantic cod (Gadus morhua) collected from the southern part of the Gotland Basin-the Baltic Sea (2010-2017).

Eight nuclear abnormalities of genotoxicity and cytotoxicity were studied in peripheral blood erythrocytes of herring (Clupea harengus membras), flounder (Platichthys flesus), and Atlantic cod (Gadus morhua) sampled (2010-2017) from the Polish and the Lithuanian Exclusive Economic Zones (EEZ) in the Baltic Sea. At all study stations, total genotoxicity (∑Gentox) was found to be higher than total cytotoxicity (∑Cytox). A significant time-related decrease in genotoxicity was detected in the Lithuanian EEZ (2015-2017), while in the Polish EEZ (2014-2016), the opposite tendency was revealed.

Correction to: Prevalence and phylogenetic analysis of hepatitis E virus in pigs, wild boars, roe deer, red deer and moose in Lithuania.

Following publication of the original article [1], we have been notified of a typing error in the "Discussion" section.

Genotoxic, cytotoxic, and neurotoxic responses in Anodonta cygnea after complex metal mixture treatment.

Environmental effects associated with the release of various metals even at maximum permissible concentrations (MPC) to the aquatic ecosystems are evident. In the present work, time-dependent increase in accumulated metals amount in gills of Anodonta cygnea after exposure to complex metal (Zn 0.1, Cu 0.

Genotoxic and cytotoxic effects of 50 Hz 1 mT electromagnetic field on larval rainbow trout (Oncorhynchus mykiss), Baltic clam (Limecola balthica) and common ragworm (Hediste diversicolor).

The aim of this research was to assess genotoxicity and cytotoxicity responses in aquatic animals exposed to 50 Hz 1 m T electromagnetic field (EMF). Rainbow trout (Oncorhynchus mykiss) at early stages of development were exposed to EMF for 40 days, whereas marine benthic invertebrates - the common ragworm Hediste diversicolor and the Baltic clam Limecola balthica - for 12 days. To define genotoxicity and cytotoxicity responses in selected animals, assays of nuclear abnormalities in peripheral blood erythrocytes of O.