Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing.

The CRISPR/Cas9 technology has greatly improved genome editing in over recent years. However, several current CRISPR/Cas9 systems suffer from work-intensive cloning procedures and/or the requirement of co-transforming target cells with multiple system components simultaneously which can reduce the effectivity of such applications. Here, we present a new set of all-in-one CRISPR/Cas9 vectors that combine unique benefits of different already existent systems in order to further expand the technology's design possibilities.