Application of a porous zirconium-based MOF nanoplate as an affinity ECL platform for the detection of protein kinase activity and inhibitor screening.

Abnormal kinase expression affects phosphorylation in the human body, which is associated with numerous diseases, including cancer, diabetes mellitus, and Alzheimer's disease. In this study, we synthesized a highly stable, two-dimensional, luminescence-functionalized metal-organic framework with remarkable electrochemiluminescence (ECL) by immobilizing 9,10-Di(p-carboxyphenyl) anthracene (dca) on a zirconium cluster (dca-Zr₁₂) via a strong coordination bond between -COO⁻ and Zr⁴⁺. This novel and simple platform relies on the highly specific identification of phosphate molecules by the ultra-thin dca-Zr₁₂ nanoplate through carboxylate-Zr⁴⁺-phosphate chemistry.

A universal ratiometric method for Micro-RNA detection based on the ratio of electrochemical/electrochemiluminescence signal, and toehold-mediated strand displacement amplification.

An ultra-selective and reproductive ratiometric platform was introduced based on the ratio of Ru(phen) electrochemiluminescence (ECL) signal and methylene blue (MB) electrochemistry (EC) signal, which was amplified using a specific and efficient toehold-mediated strand displacement (TMSD). The stable DNA nanoclews (NCs) were efficiently loaded with MB (MB-NCs) as EC signal tags after being synthesized utilizing a simple rolling circle amplification reaction. Besides, TiC-based nanocomposite could apply as a superb carrier for both Ru(phen) and gold nanoparticles (TiC-Au-Ru), resulting in a nearly constant ECL internal reference to eliminate the possible interferences.