Publications by authors named "Milad Rashidifard"
Molecular data should be combined with morphological data to enhance the reliability of phylogenetic and diagnostic studies on nematodes. In this study, the citrus nematode collected from citrus orchards in different localities in Fars province, southern Iran, was characterized using the partial sequencing of ITS rDNA, D2-D3 of 28S rDNA and COI mtDNA genes. We also morphometrically characterized the second-stage juveniles (J2) and male specimens.
View Article and Find Full Text PDFPlant-parasitic nematodes are one of the main biotic factors limiting agricultural production worldwide, with root-knot nematodes ( spp.) being the most damaging group. This study was conducted to evaluate the efficacy of soil microbiomes, associated with various subtropical fruit trees, on the management of a population.
View Article and Find Full Text PDFA newly recovered population of the genus from a dead maritime pine wood sample in Potchefstroom, South Africa, representing a new species, named n. sp., is herein described and illustrated based on morphological and molecular data.
View Article and Find Full Text PDFRoot-knot nematodes (RKNs) are one of the most important plant-parasitic nematodes of cereal crops in sub-Saharan Africa. This study was designed to evaluate the rotation effects of different cultivars of sainfoin (Esparsette, Perly, Taja and Visnovsky), soybean (DM-5953-RSF) and alfalfa (BAR 7) with maize (P-2432-R), on a population, compared to monoculture maize. The results showed that sainfoin (Perly and Esparsette) and alfalfa had significantly ( ≤ 0.
View Article and Find Full Text PDFn. sp. is described from South Africa and illustrated using morphological, morphometric, and molecular techniques.
View Article and Find Full Text PDFArticle Synopsis
- Two populations of a previously unidentified species were found in compost from gardens in Potchefstroom, South Africa, marking the first report of this species in the country.
- The specimens were analyzed using morphological, morphometric, and molecular techniques, revealing notable measurements and structural characteristics, including a specific reproductive system and genital features in males.
- Phylogenetic analysis showed a close relationship between the South African populations and an Iranian population of the same species based on genetic sequencing data.
Article Synopsis
- In the summer of 2018, a new species of nematodes was discovered in maize root samples from Stampriet, Namibia, and described using various scientific methods.
- The females of this nematode population measured an average of 1,100 µm in body length, while males were shorter at 925 µm, with detailed observations on their physical features.
- This research marks the first molecular characterization of this nematode species in Africa, highlighting its close genetic relationship with another species based on DNA analysis.
Article Synopsis
- A new species is identified and described based on its unique physical and molecular traits, including a short stylet and specific pharyngeal features.
- The species exhibits distinct morphological characteristics such as a prominent cuticle, specific tail shape, and absence of males, differentiating it from previously known species in its genus.
- Molecular analyses confirm its classification within the genus and provide insights into the biogeography of the region it was discovered in (South Africa).
Accurate identification of Meloidogyne spp. is crucial and the first step to apply suitable management strategies to combat these nematode pests. Perineal-pattern morphology of female specimens is one of the most common characteristics used for identification.
View Article and Find Full Text PDFArticle Synopsis
- Meloidogyne species, particularly M. enterolobii, cause significant crop losses and can overcome certain plant resistance genes, highlighting the need for deeper genetic understanding of these nematodes.
- The study utilized genotyping-by-sequencing (GBS) and Pool-Seq methods to analyze genetic relationships among South African populations of M. enterolobii, M. incognita, and M. javanica, identifying 653 common single nucleotide polymorphisms (SNPs).
- Results revealed that M. enterolobii is genetically distinct from M. javanica, while sharing some links with M. incognita; specific alleles in M. enterolobii were also found to