Solid-phase microextraction- probe electrospray ionization devices for screening and quantitating drugs of abuse in small amounts of biofluids.

Probe electrospray ionization (PESI) is an ambient ionization mass spectrometry technique (AIMS) that is primarily used in qualitative studies, though researchers have recently combined it with sample preparation for the quantitative analysis of various analytes in biological matrices. This study presents a method that integrates solid-phase microextraction with PESI for direct coupling to a triple quadrupole mass spectrometer, and examines its ability to quantitate drugs of abuse. Intra- and inter-probe reproducibility experiments were conducted to assess the stability and reproducibility of the extraction-phase-coated PESI probes (coating length: 2 mm; coating thickness: 6.

Comprehensive Analysis of Multiresidue Pesticides from Process Water Obtained from Wastewater Treatment Facilities Using Solid-Phase Microextraction.

A novel magnetic blade spray-tandem mass spectrometry (MBS-MS/MS) assay was developed and optimized, and its performance was characterized for the analysis of 204 pesticides from wastewater treatment facility (WWTF) process water. These results were compared and experimentally validated with an untargeted, high-resolution MS (HRMS) approach that employed liquid chromatography (LC)-amenable thin-film microextraction (TFME) devices to further elucidate the fate of pesticides through the WWTF process. As a result of our optimizations, we report an optimized workflow with an extraction time of 10 min, 150 μg of magnetic HLB particles, and 5 s of desorption.