Publications by authors named "Mikolaj Dziurzynski"

Article Synopsis
  • The study evaluates whole-genome sequencing (WGS) as a method for detecting multi-drug resistant Mycobacterium tuberculosis strains in Poland and Lithuania, highlighting its effectiveness compared to traditional drug susceptibility testing (DST).
  • Researchers analyzed 208 MTBC strains and found a 5.3% discordance rate among the results from various resistance detection methods, including WGS and molecular assays.
  • Key findings indicated that resistance mutations mainly occurred in the rpoB gene, with a consistent MIC distribution for rifampicin, suggesting that WGS can provide reliable insights but may not always align perfectly with conventional techniques.
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Gray mold, caused by is a major cause of post-harvest rot of fresh fruits and vegetables. The utilization of selected microorganisms as biocontrol agents is a promising alternative to effectively control gray mold on tomatoes. The current study was conducted to explore potential biocontrol mechanisms of the strain to control infections on post-harvest tomatoes.

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Spoligotyping is one of the molecular typing methods widely used for exploring the genetic variety of Mycobacterium tuberculosis. The aim of this study was to compare the spoligoprofiles of M. tuberculosis clinical isolates, obtained using in vitro and in silico approaches.

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Arctic soils are constantly subjected to extreme environmental conditions such as low humidity, strong winds, high salinity, freeze-thaw cycles, UV exposition, and low nutrient availability, therefore, they have developed unique microbial ecosystems. These environments provide excellent opportunities to study microbial ecology and evolution within pristine (i.e.

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The objective of this work was to compare the quality of FMT preparations made from fresh feces with those made from feces frozen at -30°C without any pre-processing or cryopreservation additives. The research hypothesis was that such preservation protocol (frozen whole stool, then thawed and processed) is equipotent to classical fresh FMT preparation. For that, three complementary methods were applied, including: (i) culturing in aerobic and anaerobic conditions, (ii) measuring viability by flow cytometry, and (iii) next-generation sequencing.

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Extreme environmental conditions observed in polar regions create a unique ecological niche for microbial life. Bacteria living under these harsh, environmental conditions exhibit specific metabolic capabilities. In this report, we present multimetal-resistant sp.

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Article Synopsis
  • The genus Prototheca consists of unicellular, non-chlorophyll containing algae that can infect vertebrates, including humans, leading to a rare condition called protothecosis, which can be hard to treat.
  • Identification of Prototheca species is complicated due to the lack of bioinformatic tools for analyzing their DNA, alongside issues with available sequence databases that have limited records and poor annotations.
  • The Prototheca-ID is a new, user-friendly web application designed to provide quick and accurate identification of Prototheca isolates and allows users to contribute their sequence data to an open database for better research quality.
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hCDKL5 refers to the human cyclin-dependent kinase like 5 that is primarily expressed in the brain. Mutations in its coding sequence are often causative of hCDKL5 deficiency disorder, a devastating neurodevelopmental disorder currently lacking a cure. The large-scale recombinant production of hCDKL5 is desirable to boost the translation of preclinical therapeutic approaches into the clinic.

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Plasmids have the potential to transfer genetic traits within bacterial communities and thereby serve as a crucial tool for the rapid adaptation of bacteria in response to changing environmental conditions. Our knowledge of the environmental pool of plasmids (the metaplasmidome) and encoded functions is still limited due to a lack of sufficient extraction methods and tools for identifying and assembling plasmids from metagenomic datasets. Here, we present the first insights into the functional potential of the metaplasmidome of permafrost-affected active-layer soil-an environment with a relatively low biomass and seasonal freeze-thaw cycles that is strongly affected by global warming.

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Over the last 15 years, the costs of DNA sequencing have sharply fallen, effectively shifting the costs of DNA analysis from sequencing to bioinformatic curation and storage. A huge number of available DNA sequences (including genomes and metagenomes) resulted in the development of various tools for sequence annotation. While much effort has been invested into the development of automatic annotation pipelines, manual curation of their results is still necessary in order to obtain a reliable and strictly validated data.

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Cultural heritage objects constitute a very diverse environment, inhabited by various bacteria and fungi. The impact of these microorganisms on the degradation of artworks is undeniable, but at the same time, some of them may be applied for the efficient biotreatment of cultural heritage assets. Interventions with microorganisms have been proven to be useful in restoration of artworks, when classical chemical and mechanical methods fail or produce poor or short-term effects.

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Methods of stool assessment are mostly focused on next-generation sequencing (NGS) or classical culturing, but only rarely both. We conducted a series of experiments using a multi-method approach to trace the stability of gut microbiota in various donors over time, to find the best method for the proper selection of fecal donors and to find "super-donor" indicators. Ten consecutive stools donated by each of three donors were used for the experiments (30 stools in total).

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Only very recently, has it been proposed that the hitherto existing subtypes (I-VI) should be elevated, each, to a species rank. Consequently, the former subtypes have been denominated as (former type I), (II), (III), (V), and (VI). The present work extends the recently published findings by using a three-pronged computational strategy, based on the alignment fraction-average nucleotide identity, genome-to-genome distance, and core-genome phylogeny, yet essentially independent and much larger sample, and thus delivers a more refined and complete picture of the complex.

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Article Synopsis
  • This study explores a comprehensive strategy for selecting bacterial strains to improve biogas production through bioaugmentation, highlighting the importance of a genome-centric method for determining effective strains.
  • Researchers examined how the bacterial strain's origin influences its metabolic capabilities and efficiency in anaerobic digestion, using various analytical techniques.
  • The results indicate that specific bacteria isolated from sewage sludge (POC4 and POC9) were more effective in enhancing biogas production compared to a strain from an agricultural biogas plant (LPMIX5), suggesting that the isolation source significantly affects enzymatic activity and performance in different substrates.
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A literature-based, manually-curated database of PCR primers for the detection of antibiotic resistance genes in various environments was constructed (LCPDb-ARG; lcpdb.ddg.biol.

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Article Synopsis
  • * The novel bacterial strain sp. POC9, isolated from sewage sludge, has multiple enzymatic activities (lipolytic, proteolytic, cellulolytic, amylolytic) that enhance biodegradation and improve biogas production in anaerobic digestion.
  • * Genome analysis of POC9 indicates it can tolerate metals, form biofilms, and utilize toxic compounds, showing low pathogenicity risk despite having some antibiotic resistance genes, making it suitable for environmental applications.
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