Publications by authors named "Mikiko Kubo"
Article Synopsis
- The Pharmaceuticals and Medical Devices Agency (PMDA) in Japan has utilized the National Database of Health Insurance Claims (NDB) for postmarketing drug safety assessments, leveraging real-world data from over 100 million individuals since 2009.
- The article highlights PMDA's experiences and the impact of these studies on regulatory decisions, such as revising drug prescribing information and evaluating safety measures.
- It also compares NDB with another database, MID-NET, to ensure the best data source selection for effective drug safety assessments in Japan.
Purpose: The effect of a preferential inducer of 78 kDa glucose-regulated protein (GRP78)/immunoglobulin heavy-chain binding protein (BiP; BiP inducer X, BIX) against tunicamycin-induced cell death in RGC-5 (a rat ganglion cell line), and also against tunicamycin- or N-methyl-D-aspartate (NMDA)-induced retinal damage in mice was evaluated.
Methods: In vitro, BiP mRNA was measured after BIX treatment using semi-quantitative RT-PCR or real-time PCR. The effect of BIX on tunicamycin (at 2 microg/mL)-induced damage was evaluated by measuring the cell-death rate and CHOP protein expression.
Altered localization of amyloid precursor protein under endoplasmic reticulum stress.
Biochem Biophys Res Commun
June 2006
Recent reports have shown that the endoplasmic reticulum (ER) stress is relevant to the pathogenesis of Alzheimer disease. Following the amyloid cascade hypothesis, we therefore attempted to investigate the effects of ER stress on amyloid-beta peptide (Abeta) generation. In this study, we found that ER stress altered the localization of amyloid precursor protein (APP) from late compartments to early compartments of the secretory pathway, and decreased the level of Abeta 40 and Abeta 42 release by beta- and gamma-cutting.
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