[DIFFERENTIATING THE POSSIBILITIES OF DIFFERENT SEGMENTS OF THE POPULATION TO USE PAID HEALTH SERVICES AS A FACTOR OF SOCIAL TENSION].

The article is devoted to the study of the impact of the possibility of using paid medical services on the level of social tension. A sociological analysis of the differentiation between the use of paid medical services by the poor and non-poor is being carried out, which are most updated during the COVID-19 period. It is summarized that the lack of funds for the care of children's own health and health has a direct impact on the growth of social tensions.

Why human anti-Galα1-4Galβ1-4Glc natural antibodies do not recognize the trisaccharide on erythrocyte membrane? Molecular dynamics and immunochemical investigation.

Background: Human blood contains a big variety of natural antibodies, circulating throughout life at constant concentration. Previously, we have found natural antibodies capable of binding to trisaccharide Galα1-4Galβ1-4Glc (P) practically in all humans. Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.

[Liver defect and change of tolerance to low alcohol drinks at young rats].

This study was performed to understand behavior and toxic liver defect in rats in the use of low-alcohol drinks (LAD) and energy drinks (ED). Young rats quickly become accustomed to the LAD, increased tolerance to alcohol. Rats were getting a habit, an intoxicating effect was faster than at the beginning of the LAD using.

[Some regularities of dimer II formation in BODIPY-FL-labeled lipids].

Some regularities of dimer II formation from BODIPY-FL-labeled lipid probes using mono-, bis-, and tris-BODIPY-FL derivatives of gangliosides G(M1) and G(D1a) and mono- and bis-BODIPY-FL derivatives of triglycerides have been defined. BODIPY-FL-labeled glycolipids were shown in phospholipid layers to reveal a greater disposition towards dimer II formation than BODIPY-FL-labeled glycerides. The formation of dimer 11 was also shown to depend on the label position in the probe molecule.

[Synthesis and characteristics of fluorescent BODIPY-labeled gangliosides].

A series of new fluorescent-labeled gangliosides bearing the residues of acids labeled by 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) in the polar or/and apolar moiety were synthesized. These are ganglioside GM1 labeled with the residue of 4,4-difluoro-4-bora-3a,4a-diaza-5,7-dimethyl-s-indacenyl-3-propanoic BODIPY-FL-propanoic) and -indacenyl-5-pentanoic (BODIPY-FL-pentanoic) acid in the oligosaccharide moiety of the molecule, and ganglioside GD1a labeled with two residues of BODIPY-FL-pentanoic acid in the oligosaccharide moiety and also with the residue of BODIPY-FL-pentanoic acid and the residue of 4,4-difluoro-4-bora-3a,4a-diaza-5-octyl-s-indacenyl-5-pentanoic acid in the ceramide part of the molecule. Some spectral characteristics and the behavior in the model membrane systems of the synthesized probes were studied.

[The synthesis of new photoaffinity probes on the basis of ganglioside GM1].

New photoaffine probes, photoreactive derivatives of ganglioside GM1 bearing a carbene-generating diazocyclopentadien-2-ylcarbonyl group at various distances from the carbohydrate moiety in their molecules, were synthesized.

Gangliosides induce cell apoptosis in the cytotoxic line CTLL-2, but not in the promyelocyte leukemia cell line HL-60.

Gangliosides induce apoptosis in the cells of the IL-2-dependent cytotoxic mouse line CTLL-2. Upon incubation with gangliosides for 24 h, their effect resulting in appearance of apoptotic cells, falls in a series GM2 > GM3 > GM1 > GD1a > GD1b > GT1b. In the presence of rIL-2, apoptosis induced by GM1 is suppressed, whereas that induced by GM2 is enhanced (the effect of intracellular agent C2-Cer is independent of this cytokine).

[Transportation of cytotoxic liposomes to malignant cells using a carbohydrate determinant].

A method of the synthesis of lipophilic glycoconjugates (vectors) on the basis of polyethyleneglycol-containing detergent was proposed. It has been shown by flow cytofluorometry that fluorescent labeled liposomes equipped with beta-galactosyl conjugate are bound human leukosis HL-60 cells more effectively than liposomes embedded with the beta-glucosyl conjugate or vector-free liposomes. A new lipid derivative of antitumor drug rubomycin (daunorubicin), N-(rac-1,2-dioleoylglycero-3-oxalyl)rubomycin (RubDG) has been synthesized.

Conjugates of oligonucleotides with polyaromatic fluorophores as promising DNA probes.

Conjugates of pyrene and perylene with oligodeoxynucleotides were synthesized and tested as hybridisation probes. A 13-mer containing a 3-peryleneacetic acid residue attached to the 5' end through a hexamethylenediamine linker showed no response in the fluorescent spectrum upon hybridisation to the complementary sequence. At the same time, pyrene-labelled probes are sensitive to duplex formation.

[Lipid derivatives of sarcolysin, methotrexate and rubomycin].

To change the pharmacokinetic properties of the known antitumor agents sarcolysine, rubomycin, and methotrexate, their lipid derivatives were synthesized with the residues of rac-1,2-dioleoylglycerol and heptadecyl alcohol. An ether lipid analog, 3-sarcolysyl 2-methyl-1-octadecyl-sn-glycerol, with the lipid moiety being itself an antineoplastic agent was also obtained.

[A new oxa-analog of myristic acid, suppressing replication of the human immunodeficiency virus].

A series of oxaanalogs of myristic acid were synthesized and tested for antiviral activity in MT4 cells infected with human immunodeficiency virus 1 (HIV-1). The synthesized acids have no toxic effect on uninfected MT4 cells at a concentration of 100 microM. 14,14,14-Trifluoro-12-oxatetradecanoic acid substantially (by 75%) inhibits the reproduction of HIV-1.

[Gangliosides from human placenta].

Gangliosides of human placenta were studied, using biochemical methods and specific antibodies. The placenta was found to contain three types of gangliosides with oligosaccharide chains Lac, GgOse4 and nLcOse4.

[Experience with the tegretol treatment of diabetes insipidus].

The use of tegretol in a 24-hour dose of 200-600 mg in 12 patients with diabetes incipidus led to the improvement of their general condition, lesser thirst, reduction of the 24-hour diuresis and increase of specific gravity of the urine. A positive effect of prescribing tegretol together with chlorpropamide was noted in a patient with diabetes mellitus and incipidus.