[Lymphocyte reaction and humoral immune response to brain specific protein in children with pathology of the central nervous system].

Blood lymphocyte reaction to brain specific protein (BSP) in vitro and anti-brain antibodies (AB) levels were studied in newborns and infants with perinatal central nervous system (CNS) damages of various severity. A relationship between severity of CNS damages and a lymphocyte reaction to BSP as well as immuno-regulative index (IRI) changes. A combination of high AB to BSP levels with IRI elevation after cells incubation with BSP was found to be a favorable prognostic sign.

[Dynamics of morphohistochemical parameters and lipid peroxidation in the course of aging of the human cerebral hemisphere cortex].

Morphometric parameters of "neuron-capillary-glia" system, succinatedehydrogenase (SDG), NAD-diaphorase and lipid peroxidation (LPO) activities were studied in area 41 and area E of human cerebral hemisphere cortex. It was established that the aging was associated with a reduction in a portion of capillaries, decline in neuronal SDG activity and an activation of LPO. The consequences of these phenomena are the death of some part of neurons accompanied by a substitutive gliosis and an increase of glial index.

[Diagnostic significance of ferritin assay in peritoneal fluid and gastric juice].

Ferritin was assayed by immunoradiometrical procedure in peritoneal fluid (26 patients with various ovarian pathologies) and gastric juice (18 patients with stomach cancer and 28 cases of gastric ulcer disease). It was found that diagnostic significance of ferritin measurements in peritoneal fluid in ovarian pathology is compromised by inflammation. Therefore, this marker cannot be used to differentiate between malignant and benign ovarian tumor.

[Second trimester Down syndrome serum screening--results from a pilot study].

The results from a pilot prospective study--second trimester Down syndrome [DS] serum screening between 15 and 21 w.g. with two markers (alpha-fetoprotein and free bb-hCG)--were summarised.

Structure-function relationships in the beta-cell K(ATP) channel.

The ATP-sensitive potassium (K(ATP)) channel plays a key role in controlling beta-cell membrane potential and insulin secretion. The channels are composed of two subunits, Kir6.2, which forms the channel pore, and SUR1, which contains binding sites for nucleotides and sulphonylureas and acts as a channel regulator.

[Genetic analysis of mutation sy2 which causes nonhomologous meiotic synapsis in chromosomes of diploid rye Secale cereale L].

Partially nonhomologous (heterologous) synapsis of meiotic chromosomes in a spontaneous desynaptic mutant form of rye is determined by two recessive genes, sy2a and sy2b, that have independent expression and inheritance. The third gene, dominant inhibitor suppressing the mutant phenotype, has been revealed in hybrid combinations between sy2 mutants and lines segregating other meiotic mutants: sy10 (heterologous synapsis), sy1, and sy9 (asynapsis). All three genes determining desynapsis (sy2a, sy2b, and I) were shown to be nonallelic to monogenic mutations sy10, sy1, and sy9, inherited independently of them and expressed at later stages of prophase I than the sy10 gene.

[Specific immunoglobulins as effective drugs for treatment of virus infections: present state and urgent tasks in Ukraine].

The thesis about importance of specific immunoglobulin preparations in treatment of infectious diseases of viral and microbial etiology, uncontrollable infections, caused by representatives of herpes viruses family in particular, is developed in the paper. The types of immunoglobulin preparations, their advantages, technology peculiarities has been considered; the experience of application, including that of the authors have been analyzed. The necessity and expedience of intensive development of this branch of immunobiotechnology in Ukraine is proved, especially in connection with a high level of infectious morbidity in this country, concrete recommendations are given.

[The expression of mutation sy2 causing nonhomologous synapsis in meiosis of diploid rye Secale cereale L].

The cytological expression of spontaneous mutation sy2 isolated from a population of weedy rye was examined. It was demonstrated that the primary defect of meiosis in the mutant plants is nonhomologous synapsis, which occurs simultaneously with the homologous one. An electron microscope study of the synaptonemal complex (SC) at prophase I showed synaptic abnormalities that manifested as "switches" of synapting axial elements to the nonhomologous partner and the formation of foldbacks of lateral SC elements.

[Pregnancy with Rh-isoimmunization. Results of a retrospective analysis in the maternity hospital "Maichin Dom"].

Unlabelled: The aim of the study is to analyze the outcome of pregnancies complicated by Rh-isoimmunization for the period 1996-2001 and to outline the aspects of optimization of the obstetrical conduct.

Material And Methods: The current study includes 39 pregnant women with Rh-isoimmunisation to whom amniocentesis and cordocentesis was performed. All cases were analyzed using medical history, serology (indirect Coombs, PAP test), ultrasound examination, amniocentesis, cordocentesis, NST.

[Recognition of joy, anger, and fear by face expression in humans].

Behavioral and neurophysiological characteristics of a visual recognition of emotions of joy, anger, and fear were studied in 9 young healthy men and 10 women. It was shown that these emotions were identified by subjects with different rate and accuracy; significant gender differences in recognition of anger and fear were found. Recording of visual evoked potentials (VEP) from the occipital (O1/2), medial temporal (T3/4), inferior temporal (T5/6), and frontal (F3/4) areas revealed differences (related with the type of emotion) in the latencies of P150, N180, P250, and N350 waves and in the amplitude of VEP components with the latencies longer than 250 ms.

[Effect of radio wave-induced hyperthermia on microflora of the prostate in the treatment of prostatitis associated with infertility].

The influence of hyperthermia induced by radiowaves on the microflora of the prostate in cases of male sterility was studied. The use of hyperthermia was shown to produce a stable decrease in bacteriospermia and the persistence potential of bacteria isolated from sperm. Hyperthermia produced a bactericidal effect not only on microorganisms contained in sperm but also on the microflora of prostatic tissues 1 cm deep.

[Colostomies in gunshot wounds of the large intestine].

The frequency and indications to colostoma formation are shown that were performed during the recent military conflicts in 40-70% of the wounded with large intestinal injuries. The authors state the indications to colostoma formation in the injuries of extra- and intra-abdominal parts of rectum and colon as well as the conditions when colostoma could be avoided. Different methods of colostoma formation and technical details are presented.

[Effectiveness of cyclosporin A in the treatment of adult patients with aplastic anemia].

Aim: To evaluate the efficacy of cyclosporin A (CyA) at different stages of immunosuppressive therapy (IST) in patients with aplastic anemia (AA).

Materials And Methods: The efficacy of CyA was studied in 56 patients with AA. The agent was orally given in an initial dose of 10 mg/kg as solution or capsules.

Molecular structure of the glibenclamide binding site of the beta-cell K(ATP) channel.

We have investigated the structure of the glibenclamide binding site of pancreatic beta-cell ATP-sensitive potassium (K(ATP)) channels. K(ATP) channels are a complex of four pore-forming Kir6.2 subunits and four sulfonylurea receptor (SUR1) subunits.

Nuclear dispositions of subtelomeric and pericentromeric chromosomal domains during meiosis in asynaptic mutants of rye (Secale cereale L.).

The nuclear dispositions of subtelomeric and pericentromeric domains in pollen mother cells (PMCs) were tracked during meiosis in wildtype and two asynaptic mutants of rye (Secale cereale L.) by means of fluorescence in situ hybridization (FISH). Homozygotes for sy1 and sy9 non-allelic mutations form axial elements during leptotene of male meiosis, but fail to form synaptonemal complexes.

[Genetic control of chromosomal synapsis in Secale cereale L. rye meiosis: sy19 gene, causing heterologous synapsis].

Analysis of manifestation and inheritance of a new mutation inducing irregular synapsis in rye showed that abnormal phenotype is determined by a recessive allele of the sy19 gene. In the homozygotes for this mutation, even at the light microscopic level, abnormal formation of bivalents is already observed at pachytene-diakinesis. At metaphase I, the univalent frequency varies from 0 to 14; in a few cells, multivalent associations of chromosomes, which are not clearly oriented in the spindle, are detected.

Evoked cerebral cortex activity in the human brain in conditions of the active and passive perception of facial expressions.

Visual evoked potentials were recorded in the occipital, parietal, central, frontal, and posterior temporal areas of the cortex in 22 healthy right-handed subjects during passive observation and recognition of emotionally positive, negative, and neutral facial expressions. Recognition of emotions was associated with significantly higher levels of cortical activity than was passive observation; this was reflected as increases in the amplitudes of the negative waves N1, N2, and N3, along with decreases in the latencies of the N1, P2, and N2 waves. The latencies of the N3 and P3 waves, which are associated with the later stages of information processing, increased as compared with those recorded during passive observation.

Visual illusions and travelling alpha waves produced by flicker at alpha frequency.

The aim of the study was to obtain some experimental evidence of the 'scanning hypothesis' that links electroencephalogram (EEG) alpha-activity with rhythmically spreading waves in the visual cortex. The hypothesis was tested in experiments with 29 healthy adults. Under flicker stimulation through closed lids with the frequency of the individual alpha-rhythm, all subjects perceived illusory visual objects (a ring or a circle, a spiral or a spiral spring, or a grid).

Investigation of the molecular assembly of beta-cell K(ATP) channels.

We have investigated the protein interactions involved in the assembly of pancreatic beta-cell ATP-sensitive potassium channels. The channels are a heterooligomeric complex of pore-forming Kir6.2 subunits and sulfonylurea receptor (SUR1) subunits.

[Neurophysiologic mechanisms of the disorders in recognition of emotions in endogenic depression].

In 46 male patients with endogenic depressions of different nosology (22 patients with affective disorder, 24 with juvenile slow-progredient schizophrenia) and 22 healthy individuals (a control group) visual evoked potentials (EPs) were registered during recognition of emotionally positive, negative or neutral facial expression. It was found that deficient recognition of emotions in depressions was determined by alteration of different stages of information analysis. In inferio-temporal and occipetal cortical areas the Eps revealed delayed primary analisis of visual information and reduction of components responsible for classification stage and decision making.

Visual evoked potentials in humans during recognition of emotional facial expressions.

Visual evoked potentials were recorded from the occipital, parietal, central, frontal, and posterior temporal areas of the cortex during recognition of emotionally positive, negative and neutral facial expressions and during passive observation in 22 right-handed healthy subjects. These studies showed that in the posterior temporal areas, the latencies of the N90, P150, and N180 waves of potentials evoked by faces with emotionally negative expressions were significantly shorter than those evoked by other types of facial stimuli. Differences were seen both on recognition and during passive observation.

[The evoked cortical activity of the cerebral hemispheres in man during the active and passive perception of facial expression].

Twenty-two right-handed subjects were asked either to identify sad, neutral, or laughing faces presented on a computer monitor or to view passively the same pictures without classification. Visual evoked potentials were recorded from F3/4, C3/4, P3/4, O1/2, and T5/6 derivations. In comparison with the passive viewing, the emotion recognition was characterized by the higher level of cortical activity reflected in the higher N1, N2, N3 amplitudes and shortened latencies of N1, P2, and N2 waves.