[Immunoenzyme analysis in a system of serologic evaluation of louse-borne typhus vaccines].

The blood sera of persons immunized with different typhus vaccines have been studied in the complement fixation test, the indirect hemagglutination test and the enzyme immunoassay. The data thus obtained indicate that the enzyme immunoassay is highly sensitive and can be universally used for the determination of antibodies to Rickettsia prowazekii after primary and booster immunization with different typhus vaccines. This method detects specific antibodies both at an early period and, which is of particular importance, at a remote period after immunization (3 years later) when complement-binding and hemagglutinating antibodies are absent.

[Incorporation of 3H-uridine into the large decidual cells of rats and their differentiation].

The antimesometrial part of rat's decidua of the 9th day of gestation was divided into three zones. Cells of either zone display their own morphological and cytochemical properties. Different rates of 3H-uridine incorporation were observed in the cytoplasm and the nucleus in cells of either zone during 5, 30, 60 and 240 minutes after precursor injection.

[Membrane potentials of different cell types of the head of the pancreas in situ in guinea pigs].

Membrane potentials of different types of cells in the pancreatic head of guinea-pigs were studied in vivo, using intracellular microelectrode technique. Cellular distribution was determined at different depths: 0-150 microns, 150-500 microns, 500-900 microns. It was established that the maximum number of low polarized cells (30.

[Mechanism of paradoxical resistance of the digastric muscle to the action of tetanus toxin].

Excitability parameters of m. masseter and m. digastricus fibers (resting minute potential--MP, action potential--AP, critical level of depolarization--CLD and rheobase currents) were determined in anesthetized rats (nembutal, 40 mg/kg) on days 3-4 and 10-15 after local intramuscular tetanus toxin injection (20 and 40 mouse Dlm, respectively).

[Mechanism of the protective action of cobamamide and leucovorin on hematopoiesis in acute blood loss].

Leucovorin and cobamamide administered alone and in combination potentiate the proliferative activity of the erythroid and myeloid cells of the bone marrow. There is lack in mutual potentiation of the drugs.

[Genetic transformation of somatic cells. XIV. Expression of gene coding for the human hepatitis B virus surface antigen in mammalian cells].

A set of recombinant plasmids with a gene encoding surface antigen of hepatitis B virus (HBsAg) is constructed. The plasmids were transfected by DEAE-dextran method into different lines of cultured cells and transient expression of the HBsAg gene was studied. The results indicate that: transcriptional enhancer of hepatitis B virus situated downstream from HBsAg gene is active in green monkey kidney cells (CVI), promoter of 5 LTR of bovine leukemia virus is trans-activated in the goat or calf cells infected with BLV.

[Functional status of the human cardiorespiratory system after 30 hours' antiorthostatic hypokinesia].

Before and after 30-day head-down tilt (-8 degrees) the cardiorespiratory function of six healthy volunteers was assessed using an exercise test (aerobic workload on the treadmill that grew to the maximal level). After hypokinesia the maximal oxygen consumption decreased by 9.9% and total oxygen debt by 23% (P less than 0.

[Denervation syndrome in the rat digastric muscle].

Excitability parameters of m. digastricus muscle fibers were investigated in anesthetized (40 mg/kg of nembutal) rats 3-5 days after denervation. The number of fibers with high polarization level was increased as was the number of fibers with low and medium level in both bellies of m.

[Action of cobamamide and leukovorin on skeletal muscle reinnervation in mechanical injury to the motor nerves].

The experiments on rats showed that cobamamide (0.5 mg/kg) and leukovorin (5 mg/kg) administered daily exerted a pronounced activating effect on the process of regeneration of mechanically injured nerve trunks. The combined administration of the drugs fails to potentiate the effect of each drug on the process of the skeletal muscle reinnervation.

[Specific antigen of granular cells of the human endometrium].

Rabbits were immunized by homogenates of endometrium obtained from women during 10-12 weeks of gestation. A specific antiserum was obtained by absorption of the crude antiserum by blood cells and plasma proteins of men with different kinds of ABO and Rh antigens, till disappearance of positive reaction with men's serum protein in the Ouchterlony test. Such an adsorbed specific antiserum continued to react with the sera of pregnant women.

Characterization of 3'----5' exonuclease associated with DNA polymerase of silkworm nuclear polyhedrosis virus.

3'----5' Exonuclease specific for single-stranded DNA copurified with DNA polymerase of nuclear polyhedrosis virus of silkworm Bombyx mori (BmNPV Pol). BmNPV Pol has no detectable 5'----3' exonuclease activity on single-stranded or duplex DNA. Analysis of the products of 3'----5' exonucleolytic reaction showed that deoxynucleoside monophosphates were released during the hydrolysis of single-stranded DNA.

[Microelectrophysiological analysis of the fiber components of the rat digastric muscle].

Membrane potential at rest (MP), action potential (AP), critical level of depolarization (CLD) and latent period (LP) of different muscle fibers were studied in two bellies of digastric muscle. Even and chaotic distribution of different muscle fibers was observed in the anterior and posterior belly, respectively. It is believed that electrophysiological data correspond to the results of histological analysis of muscle fibers in digastric muscle.