As inhabitants of soda lakes, are halo- and alkaliphilic bacteria that have previously been shown to respire with the first demonstrated Na-translocating cytochrome- oxidase (CO). The enzyme generates a sodium-motive force (Δ) as high as -270 mV across the bacterial plasma membrane. However, in these bacteria, operation of the possible Δ consumers has not been proven.
View Article and Find Full Text PDFHuman polymorphonuclear leukocytes (PMNLs, neutrophils) play a major role in the immune response to bacterial and fungal infections and eliminate pathogens through phagocytosis. During phagocytosis of microorganisms, the 5-lipoxygenase (5-LOX) pathway is activated resulting in generation of leukotrienes, which mediate host defense. In this study, a library of oligodeoxyribonucleotides (ODNs) with varying numbers of human telomeric repeats (d(TTAGGG)) and their analogues with phosphorothioate internucleotide linkages and single-nucleotide substitutions was designed.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
July 2016
A thermophilic, anaerobic, chemolithoautotrophic bacterium, strain SH388, was isolated from a shallow, submarine hydrothermal vent (Kuril Islands, Russia). Cells of strain SH388 were Gram-stain-negative short rods, 0.2-0.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
February 2016
A novel thermophilic, facultatively autotrophic bacterium, strain S2479T, was isolated from a thermal spring located in a tidal zone of a geothermally heated beach (Kuril Islands, Russia). Cells of strain S2479T were rod-shaped and motile with a Gram-negative cell-wall type. The temperature range for growth was 35-68 °C (optimum 65 °C), and the pH range for growth was pH 5.
View Article and Find Full Text PDFNucleosides Nucleotides Nucleic Acids
December 2011
(Cytosine-5)-DNA methyltransferase SsoII (M.SsoII) functions as a methyltransferase and also as a transcription factor. Chemical and photochemical crosslinking was used for exploring the structure of M.
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