Enhanced N-Glycan Profiling of Therapeutic Monoclonal Antibodies through the Application of Upper-Hinge Middle-Up Level LC-HRMS Analysis.

Therapeutic monoclonal antibodies (mAbs) are crucial in modern medicine due to their effectiveness in treating various diseases. However, the structural complexity of mAbs, particularly their glycosylation patterns, presents challenges for quality control and biosimilarity assessment. This study explores the use of upper-hinge middle-up (UHMU)-level ultra-high-performance liquid chromatography-high-resolution mass spectrometry (LC-HRMS) analysis to improve N-glycan profiling of mAbs.

Pd(II)- and Pt(II)-Assisted P-C Activation/Cyclization Reactions with a Luminescent α-Aminophosphine.

There is unceasing interest toward transformations of phosphine derivatives, which are facilitated by transition metals. We report a facile Pd(II)- and Pt(II)-assisted P-C bond cleavage in a luminescent 2-phenylbenzothiazole-based α-methylaminophosphine (, ). Specifically, reactions between and [M(COD)Cl] (M = Pd, Pt; COD = cycloocta-1,5-diene) in different solvents (methylene chloride, acetonitrile, pyridine, toluene) resulted in the formation of PPh, captured either as a bridging ligand in binuclear complexes with a {M(PPh)} moiety or as an adduct to COD in [Pt(PPhCOD)Cl].

Chromium-Lanthanide Complexes Containing the Cr═P═Cr Fragment: Synthesis, Characterization, and Computational Study.

Heterometallic complexes [Cp*Ln(μ-CO){Cr(μ-P)Cp(CO)}] [Ln = Yb (), Sm ()] were obtained in reactions of [Cp*Ln(thf)] (Ln = Sm, Yb) with [{CpCr(CO)}(μ,η-P)] (). An analogous yttrium compound [Cp*Y(μ-CO){Cr(μ-P)Cp(CO)}] () was synthesized using a three-component reaction between [Cp*Y(BPh)], , and KC. Compounds - were isolated as solvent-free crystalline phases; in the case of , the solvate was also obtained.

Europium and ytterbium complexes with o-iminoquinonato ligands: synthesis, structure, and magnetic behavior.

Complexes of divalent ytterbium (1) and europium (2) with a dianionic o-amidophenolate ligand were prepared by both the direct reduction of 4,6-di-tert-butyl-N-(2,6-diisopropylphenyl)-o-iminobenzoquinone (dpp-IQ) and the salt metathesis reaction of potassium o-amidophenolate with LnI2 (Ln = Yb, Eu). Oxidation of o-amidophenolates 1, 2 with one equivalent of dpp-IQ as well as the salt metathesis reaction of potassium o-iminosemiquinolate with LnI2 afforded ligand mixed-valent o-iminosemiquinonato-amidophenolato complexes of trivalent ytterbium (3) and europium (4). All novel complexes 1-4 were fully characterized, including the solid state structures of 1 and 2 determined by single crystal X-ray diffraction.