[Development of adaptive reactions when applying drinking mineral water and electromagnetic radiation against the background of a high-calorie diet on the model of experimental metabolic syndrome].

Objective: To identify features of development of adaptive protective reactions when applying drinking sulfate mineral water (MW) and low-intensity electromagnetic radiation of extremely high frequency (EMR EHF) against the background of a high-calorie diet and after its elimination at an early stage of development of experimental metabolic syndrome.

Material And Methods: Experiments were conducted on 40 non-linear male rats with 200-220 g. weight.

[The use of drinking mineral water and low-intensity electromagnetic radiation at an early stage of metabolic syndrome development (experimental study)].

Purpose Of The Study: To reveal the features of adaptive metabolic development, light-optical and ultrastructural reactions under the combined action of drinking mineral water (MW) and low-intensity electromagnetic radiation (EMR) of ultrahigh frequency (UHF) at an early stage of development of the experimental metabolic syndrome.

Material And Methods: The study was performed on 28 non-linear male rats weighing 180-200 g. Animals of the experimental group on the background of a high-calorie diet received at first (from the 8th day of the experiment) a course of drinking sulfate MW (24 procedures), and after its completion - a course of low-intensity EMR UHF (12 procedures), daily, 2 minutes per lumbar region in the zone projections of the adrenal glands using the Aquaton-2 device (power flux density 1 μW/cm, frequency 1000 MHz).

[Action features of the of low-intensity electromagnetic radiation at an early stage of the experimental metabolic syndrome development induced by a diet high in carbohydrates and fats].

Unlabelled: Metabolic syndrome (MS) is a complex of interrelated metabolic disorders leading to the development of abdominal obesity, hyperglycemia, insulin resistance, and dyslipidemia. To develop new methods for the prevention and treatment of MS, it seems appropriate to simulate this disease using a high-calorie diet that induces many of its symptoms. As a therapeutic and prophylactic agent, it is promising to use low-intensity electromagnetic radiation (EMR) of ultra-high frequency (UHF), which can have a stimulating effect on metabolic and regenerative processes and strengthen the mechanisms of their regulation.

46,XY,r(8)/45,XY,-8 Mosaicism as a Possible Mechanism of the Imprinted Birk-Barel Syndrome: A Case Study.

Ring chromosome 8 (r(8)) is one of the least frequent ring chromosomes. Usually, maternal chromosome 8 forms a ring, which can be lost from cells due to mitotic instability. The 8q24 region contains the imprinted gene, which is expressed from the maternal allele.

Establishment of an induced pluripotent stem cell line (ICGi025-A) from fibroblasts of a patient with 46,XY,r(8)/45,XY,-8 mosaicism.

Ring chromosomes are structural aberrations commonly associated with disease phenotype. We consider necessary to create the iPSCs with a ring chromosome 8, which can be used for disease modeling and related research. The ICGi025-A iPSCs line was obtained by the reprogramming of the skin fibroblasts from a 1-year-old boy with 46,XY,r(8)/45,XY,-8 mosaicism, developmental delay, microcephaly, dysmorphic features, diffuse muscle hypotonia, moderate proximal muscle weakness, feeding problems, and motor alalia.

[Metabolic and ultrastructural adaptation mechanisms during the primary prophylactic action of low-intensity electromagnetic radiation under normal and radiation conditions].

Background: Experimental studies have established that that low-intensity ultrahigh frequency (UHF) electromagnetic radiation (EMR) has a preventive effect under the action of radiation and other pathogenic factors, which manifests itself in the accelerated development of adaptation, in the predominance of anabolic reactions over catabolic ones, and in the strengthening of the body' structural reserves. At the same time, from the standpoint of studying the mechanisms of primary prevention, it is very important not only to identify the overall adaptation effect, but also to have an idea on the specific contribution of a physical factor used in the development of adaptation.

Aim: To identify metabolic and ultrastructural adaption parameters under the action of low-intensity UHF EMR on healthy animals and to determine their stability (safety), by using a model of radiation exposure.

[Intracellular regeneration of adrenocorticocytes in response to the prophylactic application of low-intensity electromagnetic radiation under the conditions of radiation (an experimental study)].

Background: Adaptation of the organism to the action of certain damaging factors, including radiation, is closely related to the state of the hypothalamic-pituitary-adrenal system. The important role in its activities is played by adrenocorticocytes (ACC) - the cells of the fascicular zone of the adrenal cortex. The disturbance of the structure and function of these cells can significantly impair and limit the development of the adaptive reactions in the body.

[The ultrastructure of Sertoli cells and spermatogonia in the rats exposed to radiation under conditions of therapeutic and prophylactic application of low-intensity electromagnetic emission].

Background: it has been demonstrated in various experimental studies that radiation exposure produces a negative impact on the processes of spermatogenesis associated with the disturbances of the microcirculation processes in the testes and the development of cellular and intracellular disintegration expressed as destructive changes in the cells leading to their death.

Aim: The objective of the present study was to detect the ultrastructural abnormalities in the cells of Sertoli and spermatogonia under conditions of their exposure to radiation and to identify the peculiarities of their regeneration under the influence of the therapeutic and prophylactic application of low-intensity ultra-high frequency (UHF) electromagnetic radiation (EMR) and low-intensity low-frequency magnetic field (MF).

Material And Methods: The experiments were carried out on 28 non-pedigree mature male rats with the body weight 180-220 g that were divided into four groups.

[The combined action of drinking mineral water and low-intensity electromagnetic radiation under the immobilization stress conditions (an experimental study)].

The present study carried out on white male rats in experiments with the use of biochemical, radioimmunological, and electron- microscopic methods. It was shown that the combined treatment with potable mineral water (MV) and low-intensity electromagnetic radiation (LIEMR) of ultrahigh frequency (power density less than 1 pW/cm2, the frequency about 1000 MHz) facilitated the activation of metabolic and intracellular regenerative processes in the liver and testes. One of the advantages of the combined application of MV and LIEMR over the single-factor treatment manifested itself as the weakening of stress reactions, the increase in the frequency of the plastic processes, and the more harmonious development of different forms of intracellular regeneration.

[The ultrastructural manifestations of the regenerative processes in the Sertoli cells under the action of low-intensity electromagnetic radiation in the rats subjected to stress].

The experiments on the outbred female rats using the electron microscopic technique have demonstrated that the application of ultrahigh frequency low-intensity electromagnetic radiation (LIEMR) with a flux density below 1 mCW/Cm2 and a frequency of approximately 1,000 MHz in the regime of primary prophylaxis and therapeutic-preventive action suppressed the development of the post-stress pathological ultrastructural changes and increased the activity of the regenerative processes in the Sertoli cells. It was shown that the developing adaptive and compensatory changes in the Sertoli cells most frequently involve the energy-producing structures (mitochondria) that undergo the enlargement of their average and total dimensions. Simultaneously, the amount of granular endoplasmic reticulum and the number of ribosomes increased while the intracellular links between the organelles strengthened and the reserve potential of the cells improved.

[The application of low-intensity electromagnetic radiation under immobilization stress conditions (an experimental study)].

The experiments carried out on outbred male white rats with the use of optical, electron-microscopic, biochemical, and radioimmunological methods have demonstrated that the application of low-intensity electromagnetic radiation (LI-EMR) with a flow density of 1 mcW/cm2 and a frequency of around 1,000 MHz both in the primary prophylaxis regime and as the therapeuticpreventive modality arrested the development of post-stress disorders in the rat testicles, liver, and thymus; moreover, it promoted activation of the adaptive, preventive, and compensatory processes. The data obtained provide a rationale for the application of low-intensity electromagnetic radiation to protect the organism from negative effects of stressful factors.

[The influence of drinking mineral water and magnetic field on the development of compensatory-adaptive reactions in rat testicles under immobilization stress conditions].

The experiments were carried out on the white male rats using light and electron microscopy in combination with biochemical methods. It was shown that the combination of consumption of drinking sulfate mineral water (MW) and application of a magnetic field following immobilization stress promotes the development of compensatory-adaptive processes in the rat testicles to a greater degree than drinking mineral water alone. An important contribution to the improvement of spermatogenesis comes from the restoration of structural and functional integrity of the components performing the barrier function in the testicles.

[The application of drinking mineral water in combination with zinc and silicon in rats undergoing immobilization stress].

The experiments carried out on male rats have demonstrated that the primary prophylactic application of drinking sulfate mineral water (MW) in combination with such microelements as zinc and silicon results in a more pronounced decrease of the magnitude of alterations in the liver, thymus, and blood hormonal spectrum under the action of immobilization stress than the application of mineral water alone. This effect is believed to be due to the activation of the organisms' adaptive reactions that enhances its resistance to stress.

[Prevention of early post-stress disorders in rat testicles under effect of drinking sulfate mineral water containing zinc and silicium].

Experiments performed on outbred male rats have demonstrated that consumption of drinking sulfate mineral water containing microelements (zinc and silicium) considerably slows down the development of stress-induced pathological changes in the testicles compared with the action of pure mineral water containing no admixtures. This finding confirms the important role of zinc and silicon in combination with the ion-salt base of mineral water in the enhancement of the efficacy of the adaptive and protective reactions in the testicles during the early post-stress period.

[The use of drinking sulfate mineral water in combination with laser and magnetic-laser irradiation for primary prophylaxis of post-radiation disorders(experimental study)].

Experiments on laboratory rats have demonstrated that prophylactic use of drinkable sulfate mineral water in combination with laser and magnetolaser irradiation of adrenal glands produces a radioprotecive effect that was especially well apparent in the liver and less so in the testes. Simultaneously, the functional activity of the thymus decreased. Protection of the liver by the combination of the above factors was more pronounced than the effect of each of them applied separately.

[Combined effect of musically-modulated electrical current and mineral drinking water from Khadyzhensky spring in experimental atherosclerosis].

Male rats with experimental atherosclerosis drank mineral water (Khadyzhensky spring) and were exposed to music-modulated electric current. This combined treatment showed synergism of physical (current) and balneological (mineral water) factors providing lipolytic, antioxidant, stress-limiting and antiinflammatory intravascular effects and recovery of microcirculatory processes.

[Histogenetic, metabolic, and immunologic aspects of the effect of infrared laser radiation on injured skeletal muscles from irradiated and nonirradiated rats].

Using biochemical, histological, morphometric and cytogenetic methods, it was shown that low-intensive infrared laser radiation (total dose 3.6 J/cm2), applied to the injured rat skeletal muscles, stimulated metabolism and regeneration more efficiently in the muscles locally exposed to 20 Gy X-rays compared to the unexposed muscles. The laser irradiation promoted postradiative recovery in bone marrow cells, but did not provide normalization in thymus lymphocyte activity.

[Experimental study of the role of essential trace elements of mineral waters in prevention of atherosclerosis risk factors].

Hormonal and lipid spectra of the blood serum; antioxidant, biosynthetic, microcirculatory and elastic-inhibitory activity in the myocardium and liver; proliferative and genetic activity in the thymus; microcirculation and destruction in rats with atherosclerosis drinking Khadyzhenskaya mineral water and solution of natural macro- and trace elements in the spring water (temarox) in two dilutions. It was found that elimination of atherosclerosis risk factors in rats was most complete in a course temarox drinking in dilution 1/8000.

[Effect of infrared radiation and combination of it with constant and alternating magnetic fields on the recuperative processes in the thymus, liver, and blood of stressed rats].

Changes in thymic cells activity in stressed animals were compared to the level of 11-oxycorticosteroids in the blood and antioxidant hepatic activity in exposure of the hepatic projection region and solar plexus to varying doses of infrared radiation and its combination with constant or alternating magnetic fields. It was shown that combined action of constant or variable (pulsed with negative semiperiods) magnetic fields and infrared radiation in the above exposure promotes optimal conditions for regeneration in stressed rats.

[Experimental study of the effect of pulsed infrared laser radiation on skeletal muscle regeneration after mechanical trauma].

Impulse infrared laser radiation (0.89 microm) was examined for effect on regeneration of cross-cut musculus gastrocnemius in adult rats (ten 3-min exposures, 2-3 procedures weekly for one month after the operation, modulating frequency 10 Hz, total course dose 3.6 J/cm2).

[The physiological and physicochemical mechanisms of the therapeutic and prophylactic actions of a musically modulated electrical current in experimental atherosclerosis].

In experimental atherosclerosis it is shown that frontomastoid course application of music-modulated electric current prevents oxidant modification of low density lipoproteins in the myocardium, cerebral cortex and liver. This antioxidant effect eliminates one of the principal risk factors of atherosclerosis.

[Recovery processes in the cerebral cortex, myocardium and thymus of rats with experimental atherosclerosis exposed to low-frequency electromagnetic fields on the head].

Studies of animals with experimental sclerosis has shown that a course of 10 procedures of alternative magnetic field (AMF) (50 Hz, 30 mT, 3 min daily) promotes partial recovery of the lipid spectrum and corrects vasomotor-metabolic disturbances in the cerebral cortex, myocardium and thymus caused by atherosclerosis. Combination of AMF with constant magnetic field in the same regime and location does not produce a hypolipidemic effect in atherosclerotic animals and this, in combination with increased vascular permeability may aggravate the condition. Activated microcirculation, antioxidant and antiproteinase effects in activation of biosynthetic processes in the cerebral cortex reflect inhibition in the CNS in this combined effect and create conditions for a hypotensive effect.

[Activity of the genome of cardiomyocytes as an indicator of the development of adaptive changes in the myocardium following exposure of the central nervous system to electromagnetic fields].

Methods of cardiomyocyte nuclei isolation from the myocard homogeneous mixture, and of cardiomyocyte genome activity estimation were elaborated. In the experiments with hyperlipoproteidemic rats, cardiomyocyte genome activity was shown to reflect the primary adaptive changes in the myocard, and to serve a reliable index of their influence on the CNS regulatory centres exposed to electromagnetic field, which is used for hyperlipoproteidemia treatment. The cardiomyocyte genome activity was used to distinguish between three types of development of adaptive reactions in the myocard.

[Changes in the proteinase-inhibitor system of rats with hyperlipoproteinemia during transcerebral exposures to a 100-Hz-frequency pulse current and to an ultrahigh-frequency field].

Experiments on 36 male rats with experimental hyperlipoproteinemia demonstrated that transcerebral exposure to impulse current (100 Hz, 2mA) aggravates atherogenic alterations, provokes hyperactivation of kallikrein-kinin system and unbalance of elastase inhibitory activity in the serum and myocardium. The latter may contribute to better vascular permeability for low-density lipoproteins, to development of edema of vascular intima, lability of cellular and lysosomal membranes with hydrolysis of elastine and collagen fibers of myocardial vessels and other organs. Transcerebral exposure to electromagnetic UHF field (40.