Publications by authors named "Mike Minh Fernandez"
The specific recognition of splice signals at or near exon-intron junctions is not explained by their weak conservation and instead is postulated to require a multitude of features embedded in the pre-mRNA strand. We explored the possibility of 3D structural scaffold of AdML-a model pre-mRNA substrate-guiding early spliceosomal components to the splice signal sequences. We find that mutations in the non-cognate splice signal sequences impede recruitment of early spliceosomal components due to disruption of the global structure of the pre-mRNA.
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- The study focuses on how specific protein factors recognize splice sites in mRNA, particularly highly degenerate ones, by binding to single-stranded exonic splicing motifs while lacking detailed understanding of their secondary structures.
- Analysis of mouse transcriptomes reveals that retained introns are flanked by a base-paired segment upstream and a single-stranded exonic segment downstream, with upstream loops containing splicing enhancers that are crucial for proper splicing.
- Substituting or hybridizing these upstream loops hinders splicing, indicating that the structure of exonic segments can regulate alternative splicing by affecting the accessibility of necessary splicing factors.