Characterization of Salmonella typhimurium YegS, a putative lipid kinase homologous to eukaryotic sphingosine and diacylglycerol kinases.

Salmonella typhimurium YegS is a protein conserved in many prokaryotes. Although the function of YegS is not definitively known, it has been annotated as a potential diacylglycerol or sphingosine kinase based on sequence similarity with eukaryotic enzymes of known function. To further characterize YegS, we report its purification, biochemical analysis, crystallization, and structure determination.

The Tees Confidentiality Model: mechanisms for implementing the sealed envelope.

This paper offers mechanisms capable of implementing the authorization functionality to be supported by the NHS Care Records Service. The patient-confidentiality model for the Care Records Service includes restricting access to data by placing the data in a Sealed Envelope; providing access to data based on Legitimate Relationship, and other concepts; and the overriding of access restrictions in extraordinary or emergency situations. We informally show through examples how the Tees Confidentiality Model, a sophisticated model of authorization, can be used to implement Care Records Service authorization functionality to the level currently proposed, and also to much greater levels if they ever were to be required.