Publications by authors named "Mike Kareh"
Article Synopsis
- - NHEJ and HR are key DNA repair methods for double-strand breaks, with MMEJ serving as a backup; recent research highlights important roles for the 9-1-1 complex and RHINO in MMEJ.
- - RHINO, which accumulates during mitosis and is phosphorylated by PLK1, plays a crucial role in guiding polymerase θ to double-strand breaks for repair.
- - The study shows that MMEJ can repair lingering double-strand breaks from S phase during mitosis, suggesting a complex interaction with PARP inhibitors and a synthetic lethal relationship between certain genes and repair pathways.
DNA double-strand breaks (DSBs) are toxic lesions that can lead to genome instability if not properly repaired. Breaks incurred in G1 phase of the cell cycle are predominantly fixed by non-homologous end-joining (NHEJ), while homologous recombination (HR) is the primary repair pathway in S and G2. Microhomology-mediated end-joining (MMEJ) is intrinsically error-prone and considered a backup DSB repair pathway that becomes essential when HR and NHEJ are compromised.
View Article and Find Full Text PDFMutations in the telomere-binding protein POT1 are associated with solid tumors and leukemias. POT1 alterations cause rapid telomere elongation, ATR kinase activation, telomere fragility, and accelerated tumor development. Here, we define the impact of mutant alleles through complementary genetic and proteomic approaches based on CRISPR interference and biotin-based proximity labeling, respectively.
View Article and Find Full Text PDFArticle Synopsis
- Sea cucumbers are recognized for their potential health benefits, and researchers have evaluated their anti-cancer and anti-inflammatory properties by examining their ethanolic extracts and aqueous fractions.* -
- In experiments involving human breast cancer cells (MDA-MB-231), the sea cucumber extracts significantly reduced cell growth by over 50% without being toxic, particularly in both two-dimensional and three-dimensional cultures.* -
- The study also found that these extracts inhibited the invasive characteristics of the cancer cells and diminished the secretion of inflammatory markers from certain activated immune cells, highlighting their potential therapeutic effects.*
Article Synopsis
- Despite advancements in cancer treatments, it remains a leading cause of death, highlighting the importance of exploring plant-derived compounds as effective and less toxic therapies.
- Over 3,000 plant species have shown potential in treating cancer, with around 30 compounds currently undergoing clinical trials to determine their efficacy.
- This paper focuses on alternative non-apoptotic cell death mechanisms triggered by various plant-based compounds, like resveratrol and berberine, which could reveal new pathways for cancer treatment development.