Sequential multiple assignment randomised trial to develop an adaptive mobile health intervention to increase physical activity in people poststroke in the community setting in Ireland: TAPAS trial protocol.

Introduction: Stroke is the second-leading cause of death and disability globally. Participation in physical activity (PA) is a cornerstone of secondary prevention in stroke care. Given the heterogeneous nature of stroke, PA interventions that are adaptive to individual performance are recommended.

Combined use of stable isotopes and hydrochemical characteristics to determine streamflow sources in the Jonkershoek catchment, South Africa.

The stable isotopes of water (O, H) and other hydrochemical properties were used to assess seasonal changes between sources of stream water and flow pathways in baseflow conditions for two headwater sub-catchments (∼3 km) of the Jonkershoek, a mountainous catchment in the Western Cape of South Africa. The sub-catchments differ in land cover, one is dominated by indigenous fynbos vegetation and the other by pine plantation. Stream water, higher elevation springs, and lower elevation groundwater samples were collected monthly from January 2018 to January 2019, and were analysed for stable isotopes, electrical conductivity (EC) and pH.

Limited oxygen production in the Mesoarchean ocean.

The Archean Eon was a time of predominantly anoxic Earth surface conditions, where anaerobic processes controlled bioessential element cycles. In contrast to "oxygen oases" well documented for the Neoarchean [2.8 to 2.

The truth is in the isotopes: Authenticating regionally unique South African lamb.

Stable isotope ratios (C/C and N/N) of South African lambs from different regions were measured by isotope ratio mass spectrometry (IRMS). Homogenised and defatted meat of the Longissimus lumborum muscle was assessed. The Rûens and Hantam Karoo regions had the lowest (P≤0.

Isotope (δC, δN, δH) diet-tissue discrimination in African grey parrot Psittacus erithacus: implications for forensic studies.

Diet-tissue isotopic relationships established under controlled conditions are informative for determining the dietary sources and geographic provenance of organisms. We analysed δC, δN, and non-exchangeable δH values of captive African grey parrot Psittacus erithacus feathers grown on a fixed mixed-diet and borehole water. Diet-feather ΔC and ΔN discrimination values were +3.

Effects of nutrient levels and average culture pH on the glycosylation pattern of camelid-humanized monoclonal antibody.

The impact of operating conditions on the glycosylation pattern of humanized camelid monoclonal antibody, EG2-hFc produced by Chinese hamster ovary (CHO) cells has been evaluated by a combination of experiments and modeling. Cells were cultivated under different levels of glucose and glutamine concentrations with the goal of investigating the effect of nutrient depletion levels and ammonia build up on the cell growth and the glycoprofiles of the monoclonal antibody (Mab). The effect of average pH reduction on glycosylation level during the entire culture time or during a specific time span was also investigated.

Fragmentation of negative ions from N-linked carbohydrates, part 4. Fragmentation of complex glycans lacking substitution on the 6-antenna.

Negative ion CID spectra of N-linked glycans released from glycoproteins contain many ions that are diagnostic for specific structural features such as the detailed arrangement of antennae and the location of fucose residues. Identification of such ions requires reference glycans that are often difficult to acquire in a pure state. The recent acquisition of a sample of N-glycans from a patient lacking the enzyme N-acetylglucosaminyltransferase-2 provided an opportunity to investigate fragmentation of glycans lacking a 6-antenna.

Dispositional pessimism predicts delayed return to normal activities after inguinal hernia operation.

Background: The traditional outcome measure to assess effectiveness of inguinal hernia operation was recurrence. Open mesh repair has reduced recurrence rates and attention is now turning to outcomes other than recurrence. The factors influencing the large variation in reported times of resumption of normal activities after inguinal hernia repair are many and diverse.