Publications by authors named "Mika Yamashita"

Objective: Antimicrobial photodynamic therapy (aPDT) is considered a potential treatment for biofilm infections, which have become an increasing health issue because of the rise in antimicrobial resistance. This study aimed to evaluate the bactericidal effect of aPDT using indocyanine green-loaded nanospheres with chitosan coating (ICG-Nano/c) against polymicrobial periodontal biofilms.

Methods: Composite biofilms of Porphyromonas gingivalis and Streptococcus gordonii were constructed in 96-well plates, and aPDT with ICG-Nano/c and an 810 nm diode laser was performed either by direct irradiation or transmitting irradiation through a 3-mm-thick gingival model.

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The aim of this study is to examine the effects of ageing on dermal fibroblast heterogeneity based on samples obtained from the same donor. We used a dermal fibroblast lineage (named ASF-4 cell lines) isolated from the inner side of the upper arm of a healthy male donor over a 35-year period, beginning at 36 years of age. Because clonal analysis of ASF-4 cell lines demonstrated a donor age-dependent loss of proliferative capacity and acquisition of senescent traits at the single-cell level, cultured cells frozen at passage 10 at ages 36 and 72 years were subjected to single-cell RNA sequencing.

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Article Synopsis
  • Senile lentigo, also known as age spots, are dark patches on the skin caused by long-term UV exposure and involve the accumulation of melanin granules in skin cells.
  • The research highlights the importance of studying the complex relationship between melanocytes (cells producing melanin) and keratinocytes (skin cells) in a three-dimensional context, as traditional two-dimensional studies have limitations.
  • Advanced microscopy techniques revealed that melanocyte dendritic processes are more branched and encapsulate nearby keratinocytes, facilitating the efficient transfer of melanosomes throughout the skin layers.
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Glucosylceramide (GlcCer) is derived from several plants, such as rice, maize, and wheat, and has been reported to retain moisture by functioning as a barrier between the epidermis and the environment. However, there is insufficient research on the effect of GlcCer on dermal elasticity and wrinkles. In this study, we investigated the effects of torula yeast extract and torula yeast-derived GlcCer on dermal elasticity.

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Trichomonad is a type of flagellate that parasitizes the human oral cavity, intestine, or vagina. However, respiratory infection is rare. We report a patient with pyothorax related to Tetratrichomonas species, which is the first reported case in Japan.

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We experienced a case of right median nerve palsy at the distal forearm following abdominal surgery. We postulate that the cause of the median nerve palsy is overextension of the wrist by the inappropriate fixation with a holder. The patient was a 46-year-old man with habit of smoking receiving low-anterior resection of the rectum under general and epidural anesthesia in lithotomy position.

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Stent thrombosis during perioperative period is a critical complication for patients treated with drug-eluting stent (DES). We experienced a case of late DES thrombosis 5 years after initial implantation. A 48-year-old man with familial hyperlipidemia, angina pectoris and chronic pulmonary emphysema, was diagnosed with esophageal carcinoma, and scheduled for esophagectomy.

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Article Synopsis
  • A case involving a 69-year-old woman undergoing spine surgery revealed intraoperative kinking of her endotracheal tube (ETT) while in a prone position.
  • The kinking occurred possibly due to inadequate technique during the use of the Pentax-AWS Airway Scope for intubation, leading to increased airway pressures and ventilation difficulties.
  • The medical team addressed the issue by adjusting neck flexion and using a bronchoscope to reposition the ETT, ultimately ensuring stable ventilation without further complications.
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Background: Plants are the source of important products with nutritional and therapeutic value. Topical or oral administration of some plant extracts has been shown to reduce photodamage. Cacao bean and cola nut are popular edible plants that contain polyphenols and xanthine derivatives.

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Background: Ergocalciferol (VD(2)) is usually administered orally and it is metabolized to produce its biologically active metabolites in the liver and kidney. Active vitamin D is a well-known potent regulator of cell growth and differentiation.

Purpose: Active vitamin D such as 1,25-dihydroxyvitamin D(3) (1alpha,25(OH)(2)D(3)) prevents photodamage, including wrinkles and morphologic alterations.

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