Evaluation of Diagnostic Performance of Circulating microRNAs as Biomarkers of Retinal Toxicity in the Rat.

Retinal toxicity is of great concern during drug development due to the irreversibility. Circulating microRNA (miRNA) is reported to be useful for detecting retinal toxicity in rats, although there has been no assessment of the diagnostic performance with statistical analysis. Therefore, we comparatively analyzed the diagnostic performance of circulating miRNAs enriched in the retina such as rno-miR-124-3p, -183-5p, -96-5p, -182, -9a-5p, -125b-5p, -204-5p and -211-5p.

Simple propagation method for resident macrophages by co-culture and subculture, and their isolation from various organs.

Background: Resident macrophages (Mø) originating from yolk sac Mø and/or foetal monocytes colonise tissues/organs during embryonic development. They persist into adulthood by self-renewal at a steady state, independent of adult monocyte inputs, except for those in the intestines and dermis. Thus, many resident Mø can be propagated in vitro under optimal conditions; however, there are no specific in vitro culture methods available for the propagation of resident Mø from diverse tissues/organs.