Overview of extraction methods for analysis of vitamin D and its metabolites in biological samples.

In the last decade the scientific and medical community was confronted with a renewed interest in vitamin D and its metabolites, interest prompted by new discoveries regarding the association between members of the vitamin D family and a great number of physiological functions and pathological states. An impressive number of research projects have helped clear the path towards a better understanding of the functions of vitamin D and have resulted in the development of numerous methods of analysis. This review focuses on the various extraction methods used for analysis of vitamin D in research or clinical settings.

Analytical methods used in conjunction with solid-phase microextraction: a review of recent bioanalytical applications.

Integration of sampling and sample preparation with various analytical instruments is a highly desirable feature for any analytical method. This is most conveniently achieved by using microextraction techniques or various microdevices. Among these techniques, solid-phase microextraction (SPME) is particularly remarkable due to its simplicity and effectiveness.

Biocompatible solid-phase microextraction coatings based on polyacrylonitrile and solid-phase extraction phases.

The applications of solid-phase microextraction (SPME) are continuously expanding, and one of the most interesting current aspects consists of applying SPME for fast analysis of biological fluids. The goal of this study is to develop biocompatible SPME coatings that can be utilized for in vivo and in vitro extractions, in direct contact with a biological matrix such as blood or tissue. The biocompatibility of the proposed new coatings is confirmed by X-ray photoelectron spectroscopy, and their performance is tested by developing an SPME/HPLC method for analysis of verapamil, loperamide, diazepam, nordiazepam, and warfarin in buffer solutions and in human plasma.

Fast in vivo microextraction: a new tool for clinical analysis.

Background: We sought to develop a technique with the potential to partly replace current methods of analysis based on blood draws. To achieve this goal, we developed an in vivo microextraction technique that is faster than conventional methods, interferes minimally with the investigated system, minimizes errors associated with sample preparation, and limits exposure to hazardous biological samples.

Methods: Solid-phase microextraction devices based on hydrophilic polypyrrole and polyethylene glycol coatings were used for direct extraction of drugs from the flowing blood of beagle dogs, over a period of 8 h.