Publications by authors named "Miguel-Angel Perez-Pardo"
Article Synopsis
- Small scale bioreactors, like the 25-mL miniaturized stirred tank bioreactor (MSBR), are vital for speeding up bioprocess development by allowing for clear scale translations from larger reactors.
- Characterization results indicate that the MSBR can effectively mimic the performance of a larger 20-L stirred tank bioreactor regarding growth and Fab' production in E. coli.
- The combination of the MSBR with ultra scale-down centrifugation techniques offers a cost-effective strategy for designing bioprocesses that optimize both production and downstream recovery.
Host cell engineering is becoming a realistic option in whole bioprocess strategies to maximize product manufacturability. High molecular weight (MW) genomic DNA currently hinders bioprocessing of Escherichia coli by causing viscosity in homogenate feedstocks. We previously showed that co-expressing Staphylococcal nuclease and human Fab' fragment in the periplasm of E.
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