Publications by authors named "Miguel de Diego Fuertes"
Article Synopsis
- Oxford Nanopore Technologies (ONT) has introduced R10 flowcells and V14 rapid sequencing chemistry, which were evaluated for sequencing Mycobacterium tuberculosis (Mtb) DNA from clinical samples, yielding mixed results in terms of quality and quantity.
- In initial runs, sequencing generated a moderate amount of data (1.94 Gb, 214k reads), but only 49% of reads met acceptable quality standards (Phred score >8). Subsequent experiments with a clean-up step improved yield to 2.38 Gb and 66% quality, yet still fell short of optimal performance.
- The study indicates that further optimization of ONT protocols is necessary, as the data did not meet the
Background: Whole genome sequencing (WGS) holds great potential for the management and control of tuberculosis. Accurate analysis of samples with low mycobacterial burden, which are characterized by low (<20x) coverage and high (>40%) levels of contamination, is challenging. We created the MAGMA (Maximum Accessible Genome for Mtb Analysis) bioinformatics pipeline for analysis of clinical Mtb samples.
View Article and Find Full Text PDFBackground: Understanding the transmission dynamics of Mycobacterium tuberculosis (Mtb) could benefit the design of tuberculosis (TB) prevention and control strategies for refugee populations. Whole Genome Sequencing (WGS) has not yet been used to document the Mtb transmission dynamics among refugees in Ethiopia. We applied WGS to accurately identify transmission clusters and Mtb lineages among TB cases in refugee camps in Ethiopia.
View Article and Find Full Text PDF