Publications by authors named "Miguel Ramos-Leal"

Article Synopsis
  • The study introduces a novel strategy for designing immobilized enzyme derivatives (RDID) to predict protein behavior before synthesis, using mathematical algorithms and bioinformatics tools.
  • The focus is on validating the RDID approach for the covalent immobilization of laccase enzyme from Trametes maxima onto specific supports, with predictions being confirmed through experimental results.
  • Results show that the laccase-MANA-Sepharose CL 4B derivative has significantly higher activity than its counterpart (63.6% vs. 29.5%) and is effective for wastewater treatment, highlighting its potential as a biocatalyst.
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The original version of this article was revised: After publication of this article, the publisher was notified that Michael Klocke has been listed as an author without his consent.

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Microbial metagenome analysis has proven its usefulness to investigate the microbiomes present in technical engineered ecosystems such as anaerobic digestion systems. The analysis of the total microbial genomic DNA allows the detailed determination of both the microbial community structure and its functionality. In addition, it enables to study the response of the microbiome to alterations in technical process parameters.

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Since polycyclic aromatic hydrocarbons (PAHs) are mutagenic, teratogenic, and carcinogenic, they are of considerable environmental concern. A biotechnological approach to remove such compounds from polluted ecosystems could be based on the use of white-rot fungi (WRF). The potential of well-adapted indigenous Ganoderma strains to degrade PAHs remains underexplored.

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White-rot fungi (WRF) and their ligninolytic enzymes (laccases and peroxidases) are considered promising biotechnological tools to remove lignin related Persistent Organic Pollutants from industrial wastewaters and contaminated ecosystems. A high diversity of the genus has been reported in Cuba; in spite of this, the diversity of ligninolytic enzymes and their genes remained unexplored. In this study, 13 native WRF strains were isolated from decayed wood in urban ecosystems in Havana (Cuba).

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