Molecular Detection of Lymph Node Metastases with One-Step Nucleic Acid Amplification (OSNA) Pooling in Prostate Cancer: The POPCORN Study.

Pelvic lymph node dissection (PLND) is the most accurate procedure for lymph node (LN) staging in prostate cancer (PCa) patients. LN sectioning and hematoxylin and eosin (H&E) staining of at least one slice remains the gold standard for LN evaluation, potentially leading to misdetection of small metastatic focus. Entire LN analysis is possible with One-Step Nucleic Acid Amplification (OSNA) by detecting cytokeratin 19 (CK19) mRNA as a surrogate for LN invasion.

Assessing the potential of nature-based solutions as sustainable land and water management strategies in the high tropical Andean páramo ecosystem.

Nature-based solutions (NbS) are gaining attention as viable strategies for restoring water-rich ecosystems such as the páramo. However, the lack of evidence on their effectiveness, coupled with a limited understanding of their political, economic, social, technological, environmental, and legal (PESTEL) context, hinders their widespread adoption. To address this gap, we propose a multi-method framework that (i) captures PESTEL factors influencing the implementation of passive ecological restoration in páramo ecosystems as a measure of NbS and (ii) assesses its associated environmental impacts.

Perceptions of government-assisted payors: a survey and analysis of American Shoulder and Elbow Surgeons current Medicare, Medicare Advantage, and Medicaid practice allocation.

Introduction: Significant discrepancy and variance exist in the United States health care system with regard to patient access to medical care based on a patient's insurance type, whether that be government-assisted or a private insurer. There are currently 3 major government-assisted insurance programs, Medicare, Medicare Advantage, and Medicaid, each of which has its own patient mix and regulatory processes that govern care delivery. The purpose of this study was to evaluate the current perceptions of shoulder and elbow surgeons surrounding practice patterns and barriers to access for patients whose primary insurance is a government-assisted payor.

Biocatalytic reductive amination as a route to isotopically labelled amino acids suitable for analysis of large proteins by NMR.

We demonstrate an atom-efficient and easy to use H-driven biocatalytic platform for the enantioselective incorporation of H-atoms into amino acids. By combining the biocatalytic deuteration catalyst with amino acid dehydrogenase enzymes capable of reductive amination, we synthesised a library of multiply isotopically labelled amino acids from low-cost isotopic precursors, such as HO and NH. The chosen approach avoids the use of pre-labeled H-reducing agents, and therefore vastly simplifies product cleanup.

Identification and characterization of transcribed enhancers during cerebellar development through enhancer RNA analysis.

Background: The development of the brain requires precise coordination of molecular processes across many cell-types. Underpinning these events are gene expression programs which require intricate regulation by non-coding regulatory sequences known as enhancers. In the context of the developing brain, transcribed enhancers (TEs) regulate temporally-specific expression of genes critical for cell identity and differentiation.

Study of Metakaolinite Geopolymeric Mortar with Plastic Waste Replacing the Sand: Effects on the Mechanical Properties, Microstructure, and Efflorescence.

In this study, the production of a mortar was proposed in which plastic waste replaced sand by 0%, 50%, and 100% in order to create a sustainable alternative for construction. The performance of the mortars was tested with two types of activators, one with NaOH, as a simple activator, and the other with NaOH and NaSiO, as a compound activator. The effects of the LDPE plastic bag waste and the activators on compressive strength, porosity, microstructure analysis, and efflorescence formation were correlated and discussed.

Cryobanking European Mink () Mesenchymal Stem Cells and Oocytes.

The European mink () is one of Europe's most endangered species, and it is on the brink of extinction in the Iberian Peninsula. The species' precarious situation requires the application of new ex situ conservation methodologies that complement the existing ex situ and in situ conservation measures. Here, we report for the first time the establishment of a biobank for European mink mesenchymal stem cells (emMSC) and oocytes from specimens found dead in the Iberian Peninsula, either free or in captivity.

Cochlear ribbon synapse maturation requires Nlgn1 and Nlgn3.

Hearing depends on precise synaptic transmission between cochlear inner hair cells and spiral ganglion neurons through afferent ribbon synapses. Neuroligins (Nlgns) facilitate synapse maturation in the brain, but they have gone unstudied in the cochlea. We report and knockout (KO) cochleae have fewer ribbon synapses and have impaired hearing.

Temporal analysis of enhancers during mouse cerebellar development reveals dynamic and novel regulatory functions.

We have identified active enhancers in the mouse cerebellum at embryonic and postnatal stages which provides a view of novel enhancers active during cerebellar development. The majority of cerebellar enhancers have dynamic activity between embryonic and postnatal development. Cerebellar enhancers were enriched for neural transcription factor binding sites with temporally specific expression.

The Cerebellar Gene Database: a Collective Database of Genes Critical for Cerebellar Development.

This report presents the first comprehensive database that specifically compiles genes critical for cerebellar development and function. The Cerebellar Gene Database details genes that, when perturbed in mouse models, result in a cerebellar phenotype according to available data from both Mouse Genome Informatics and PubMed, as well as references to the corresponding studies for further examination. This database also offers a compilation of human genetic disorders with a cerebellar phenotype and their associated gene information from the Online Mendelian Inheritance in Man (OMIM) database.

Comparison of Biological Features of Wild European Rabbit Mesenchymal Stem Cells Derived from Different Tissues.

Although the European rabbit is an "endangered" species and a notorious biological model, the analysis and comparative characterization of new tissue sources of rabbit mesenchymal stem cells (rMSCs) have not been well addressed. Here, we report for the first time the isolation and characterization of rMSCs derived from an animal belonging to a natural rabbit population within the native region of the species. New rMSC lines were isolated from different tissues: oral mucosa (rOM-MSC), dermal skin (rDS-MSC), subcutaneous adipose tissue (rSCA-MSC), ovarian adipose tissue (rOA-MSC), oviduct (rO-MSC), and mammary gland (rMG-MSC).

Mesenchymal Stem Cells in Embryo-Maternal Communication under Healthy Conditions or Viral Infections: Lessons from a Bovine Model.

Bovine mesenchymal stem cells are a relevant cell population found in the maternal reproductive tract that exhibits the immunomodulation capacity required to prevent embryo rejection. The phenotypic plasticity showed by both endometrial mesenchymal stem cells (eMSC) and embryonic trophoblast through mesenchymal to epithelial transition and epithelial to mesenchymal transition, respectively, is essential for embryo implantation. Embryonic trophoblast maintains active crosstalk via EVs and soluble proteins with eMSC and peripheral blood MSC (pbMSC) to ensure the retention of eMSC in case of pregnancy and induce the chemotaxis of pbMSC, critical for successful implantation.

Evaluation of 1D and 2D Deep Convolutional Neural Networks for Driving Event Recognition.

Driving event detection and driver behavior recognition have been widely explored for many purposes, including detecting distractions, classifying driver actions, detecting kidnappings, pricing vehicle insurance, evaluating eco-driving, and managing shared and leased vehicles. Some systems can recognize the main driving events (e.g.

Predictors of mortality in hospitalized COVID-19 patients: A Mexican population-based cohort study.

Objectives: COVID-19 outbreak brings a challenge to healthcare systems. The sex, age, and cardiometabolic comorbidities have been considered risk factors for disease severity. To evaluate the association between risk factors with death as well the risk of death in hospitalized COVID-19 patients.

A Case of Fatal Catatonia in a COVID-19 Patient.

COVID-19 has been associated with numerous complications, primarily pulmonary in origin. However, there have been several neurological sequelae of COVID-19 as well, one of the rarer complications is catatonia. In this already vulnerable population, it is imperative for the early diagnosis of catatonia and starting treatment.

Nickel-Iron Hydrogenase 1 Catalyses Non-native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme Ene-reductases.

A new activity for the [NiFe] uptake hydrogenase 1 of Escherichia coli (Hyd1) is presented. Direct reduction of biological flavin cofactors FMN and FAD is achieved using H as a simple, completely atom-economical reductant. The robust nature of Hyd1 is exploited for flavin reduction across a broad range of temperatures (25-70 °C) and extended reaction times.

Embryonic Trophectoderm Secretomics Reveals Chemotactic Migration and Intercellular Communication of Endometrial and Circulating MSCs in Embryonic Implantation.

Embryonic implantation is a key step in the establishment of pregnancy. In the present work, we have carried out an in-depth proteomic analysis of the secretome (extracellular vesicles and soluble proteins) of two bovine blastocysts embryonic trophectoderm primary cultures (BBT), confirming different epithelial-mesenchymal transition stages in these cells. BBT-secretomes contain early pregnancy-related proteins and angiogenic proteins both as cargo in EVs and the soluble fraction.

Intramolecular Nicholas Reaction Enables the Stereoselective Synthesis of Strained Cyclooctynes.

Cyclic products can be obtained through the intramolecular version of the Nicholas reaction, which requires having the nucleophile connected to the alkyne unit. Here, we report the synthesis of 1-oxa-3-cyclooctynes starting from commercially available (1,3)-camphoric acid. The strategy is based on the initial preparation of propargylic alcohols, complexation of the triple bond with Co(CO), and treatment with BF·EtO to induce an intramolecular Nicholas reaction with the free hydroxyl group as nucleophile.

Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardiac scaffolds for myocardial infarction.

The administration of extracellular vesicles (EV) from mesenchymal stromal cells (MSC) is a promising cell-free nanotherapy for tissue repair after myocardial infarction (MI). However, the optimal EV delivery strategy remains undetermined. Here, we designed a novel MSC-EV delivery, using 3D scaffolds engineered from decellularised cardiac tissue as a cell-free product for cardiac repair.

E. coli Nickel-Iron Hydrogenase 1 Catalyses Non-native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme Ene-reductases*.

A new activity for the [NiFe] uptake hydrogenase 1 of Escherichia coli (Hyd1) is presented. Direct reduction of biological flavin cofactors FMN and FAD is achieved using H as a simple, completely atom-economical reductant. The robust nature of Hyd1 is exploited for flavin reduction across a broad range of temperatures (25-70 °C) and extended reaction times.

Enhanced Routing Algorithm Based on Reinforcement Machine Learning-A Case of VoIP Service.

The routing algorithm is one of the main factors that directly impact on network performance. However, conventional routing algorithms do not consider the network data history, for instances, overloaded paths or equipment faults. It is expected that routing algorithms based on machine learning present advantages using that network data.

Noise Exposures Causing Hearing Loss Generate Proteotoxic Stress and Activate the Proteostasis Network.

Exposure to excessive sound causes noise-induced hearing loss through complex mechanisms and represents a common and unmet neurological condition. We investigate how noise insults affect the cochlea with proteomics and functional assays. Quantitative proteomics reveals that exposure to loud noise causes proteotoxicity.

Catastrophic Failure of Reverse Shoulder Arthroplasty from a Broken Screw: A Case Report.

Case: A 63-year-old woman presented with pain and limited range of motion 2 years after undergoing reverse shoulder arthroplasty (RSA). She underwent revision RSA for catastrophic implant failure caused by central screw breakage, subsequent polyethylene wear, and glenosphere loosening, and achieved excellent outcomes.

Conclusion: This is a unique case of a salvage procedure with satisfactory outcomes for a rare complication of RSA.

Management of irreparable massive rotator cuff tears: a systematic review and meta-analysis of patient-reported outcomes, reoperation rates, and treatment response.

Background: There is no consensus on the treatment of irreparable massive rotator cuff tears. The goal of this systematic review and meta-analysis was to (1) compare patient-reported outcome scores, (2) define failure and reoperation rates, and (3) quantify the magnitude of patient response across treatment strategies.

Methods: The MEDLINE, Embase, CENTRAL (Cochrane Central Register of Controlled Trials), and Scopus databases were searched for studies including physical therapy and operative treatment of massive rotator cuff tears.

Novel Approaches of Nanoceria with Magnetic, Photoluminescent, and Gas-Sensing Properties.

The modification of CeO with rare-earth elements opens up a wide range of applications as biomedical devices using infrared emission as well as magnetic and gas-sensing devices, once the structural, morphological, photoluminescent, magnetic, electric, and gas-sensing properties of these systems are strongly correlated to quantum electronic transitions between rare-earth f-states among defective species. Quantitative phase analysis revealed that the nanopowders are free from secondary phases and crystallize in the fluorite-type cubic structure. Magnetic coercive field measurements on the powders indicate that the substitution of cerium with lanthanum (8 wt %), in a fluorite-type cubic structure, created oxygen vacancies and led to a decrease in the fraction of Ce species in the 3+ state, resulting in a stronger room-temperature ferromagnetic response along with high coercivity (160 Oe).