Heart-cut achiral-chiral LC-LC method development using factorial design: application to the chiral separation of ketoprofen.

A two-dimensional achiral-chiral LC-LC method in heart-cut mode for ketoprofen and its enantiomeric fraction determination was proposed. A C column was used in the first dimension, and the chiral column was an α-acid glycoprotein. The mobile phase of the chiral system was optimized by a factorial design.