Publications by authors named "Miguel G Fontela"
The HLA-B*27:05 allele and the endoplasmic reticulum-resident aminopeptidases are strongly associated with AS, a chronic inflammatory spondyloarthropathy. This study examined the effect of ERAP2 in the generation of the natural HLA-B*27:05 ligandome in live cells. Complexes of HLA-B*27:05-bound peptide pools were isolated from human ERAP2-edited cell clones, and the peptides were identified using high-throughput mass spectrometry analyses.
View Article and Find Full Text PDFThe immune regulatory receptor CD69 is expressed upon activation in all types of leukocytes and is strongly regulated at the transcriptional level. We previously described that, in addition to the promoter, there are four conserved noncoding regions () upstream of the promoter. Furthermore, we proposed that is the main enhancer of transcription.
View Article and Find Full Text PDFArticle Synopsis
- CD69 is an early marker of leukocyte activation, playing a vital role in lymphocyte migration and cytokine secretion, with expression levels varying based on lineage, activation state, and cell location.
- The study investigates the transcriptional regulation of the CD69 gene in mammals, identifying the crucial role of a specific conserved non-coding sequence (CNS2) in its expression.
- By using techniques like chromatin immunoprecipitation, the research shows that the transcription factor RUNX1 binds to CNS2, highlighting its importance in promoting CD69 transcription in hematopoietic cells.