Intragenic antimicrobial peptide Hs02 toxicity against leukemia cell lines is associated with increased expression of select pyroptotic components.

The anticancer potential of some antimicrobial peptides has been reported. Hs02 is a recently characterized Intragenic Antimicrobial Peptide (IAP), which was able to exhibit potent antimicrobial and anti-inflammatory action. In this study, we evaluate for the first time the antineoplastic potential of the Hs02 IAP using cell lines representing the main types of leukemia as cancer models.

Degree of Contamination of Gutta-Percha Points by (MRSA/MSSA) Strains.

Unlabelled: Methicillin-resistant (MRSA) is considered one of the most harmful bacteria to human health. Dentistry, like all healthcare disciplines, places great emphasis on preventing scenarios that may result in cross-infection. Although various tested and already used materials are suitable for filling the root canal system, Gutta-Percha (GP) remains the preferred and widely accepted gold standard.

Effectiveness of photodynamic therapy on treatment response and survival in patients with recurrent oral squamous cell carcinoma: A systematic review.

Background: This systematic review assessed the effectiveness of photodynamic therapy (PDT) in patients with recurrent oral squamous cell carcinoma (OSCC).

Methods: Clinical studies on recurrent OSCC treated with PDT alone were included. Combined treatment strategies were excluded.

Antiviral Action against SARS-CoV-2 of a Synthetic Peptide Based on a Novel Defensin Present in the Transcriptome of the Fire Salamander ().

The potential emergence of zoonotic diseases has raised significant concerns, particularly in light of the recent pandemic, emphasizing the urgent need for scientific preparedness. The bioprospection and characterization of new molecules are strategically relevant to the research and development of innovative drugs for viral and bacterial treatment and disease management. Amphibian species possess a diverse array of compounds, including antimicrobial peptides.

Impact of the First Wave of the COVID-19 Pandemic on Trade between Canada and the United States.

We examine how the coronavirus disease 2019 (COVID-19) pandemic has affected trade between Canada and the United States, using a novel dataset on monthly bilateral trade flows between Canadian provinces and US states merged with COVID-19 health data. Our results show that a one-standard-deviation increase in COVID-19 severity (case levels, hospitalizations, deaths) in a Canadian province leads to a 3.1 percent to 4.

Isolation and Identification of Cytotoxic Compounds Present in Biomaterial Life.

Direct pulp capping consists of a procedure in which a material is directly placed over the exposed pulp to maintain dental vitality. Although still widely used in clinical practice, previous in vitro studies found that the biomaterial Life presented high cytotoxicity, leading to cell death. This study aimed to identify the Life constituents responsible for its cytotoxic effects on odontoblast-like cells (MDPC-23).

Management of Hypercholesterolemia Through Dietary ß-glucans-Insights From a Zebrafish Model.

Consumption of lipid-rich foods can increase the blood cholesterol content. β-glucans have hypocholesterolemic effect. However, subtle changes in their molecular branching can influence bioactivity.

Effects of Adper Scotchbond 1 XT, Clearfil SE Bond 2 and Scotchbond Universal in Odontoblasts.

This study aimed to assess the cytotoxicity of commercially available adhesive strategies-etch-and-rinse (Adper Scotchbond 1 XT, 3M ESPE, St. Paul, MN, USA, SB1), self-etch (Clearfil SE Bond 2, Kuraray Noritake Dental Inc., Tokyo, Japan, CSE), and universal (Scotchbond Universal, 3M Deutschland GmbH, Neuss, Germany, SBU).

Effectiveness of photodynamic therapy on treatment response and survival of patients with recurrent oral squamous cell carcinoma: a systematic review protocol.

Objective: This review aims to systematically examine the effectiveness of photodynamic therapy for the treatment of patients with recurrent oral squamous cell carcinoma.

Introduction: Oral squamous cell carcinoma is a significant public health problem, and is the seventh most common cancer. Its incidence is mainly due to tobacco use, alcohol consumption, and HPV infection.

Effectiveness of a lifestyle weight-loss intervention targeting inactive former elite athletes: the Champ4Life randomised controlled trial.

Objectives: Many athletes struggle in managing the end of their career, often gaining weight and adopting unhealthy lifestyles. Lifestyle programmes targeting former athletes who have gained substantial fat mass (FM) postsports career are lacking. We studied the effects of the Champ4Life programme on body composition and other health-related outcomes in former elite athletes with overweight or obesity.

Methods and cell-based strategies to produce antibody libraries: current state.

Antibodies are critical components of the adaptive immune system, whose therapeutic applications have been growing exponentially in the last years. Discovery and development of therapeutic antibodies encompasses in vivo immunization, synthetic libraries, and surface display methodologies. To overcome some of their limitations, several platforms in higher eukaryotic cells have been developed.

Phase Angle Is a Marker of Muscle Quantity and Strength in Overweight/Obese Former Athletes.

Background: An increasing body of evidence indicates that the phase angle (PhA) can be applied as a marker of nutritional status, disease prognosis, and mortality probability. Still, it is not known whether PhA can be used as an indicator of muscular quantity and strength and maximal aerobic capacity in overweight/obese former highly active individuals, an understudied population. This study aimed to analyze the association between PhA with skeletal muscle mass, maximal isometric strength, and maximal aerobic capacity through VO2max, in overweight/obese and inactive former athletes.

Integrated in Silico and Experimental Approach towards the Design of a Novel Recombinant Protein Containing an Anti-HER2 scFv.

Biological therapies, such as recombinant proteins, are nowadays amongst the most promising approaches towards precision medicine. One of the most innovative methodologies currently available aimed at improving the production yield of recombinant proteins with minimization of costs relies on the combination of in silico studies to predict and deepen the understanding of the modified proteins with an experimental approach. The work described herein aims at the design and production of a biomimetic vector containing the single-chain variable domain fragment (scFv) of an anti-HER2 antibody fragment as a targeting motif fused with HIV gp41.

A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach.

Breast cancer is one of the most frequently diagnosed malignancies and common causes of cancer death in women. Recent studies suggest that environmental exposures to certain chemicals, such as 7,12-Dimethylbenzanthracene (DMBA), a chemical present in tobacco, may increase the risk of developing breast cancer later in life. The first-line treatments for breast cancer (surgery, chemotherapy or a combination of both) are generally invasive and frequently associated with severe side effects and high comorbidity.

Efficacy and Patient's Acceptance of Alternative Methods for Caries Removal-a Systematic Review.

Dental caries is a disease of dental hard tissues, considered the most common non-communicable disease worldwide. Conventional treatments for caries removal are often associated with pain and fear, so different therapeutic approaches have been developed towards more conservative and pleasant treatments. This systematic review aimed to assess the efficacy and patient's acceptance of alternative methods for caries removal compared to conventional methods.

Synthetic antibody discovery against native antigens by CRISPR/Cas9-library generation and endoplasmic reticulum screening.

Despite the significant advances of antibodies as therapeutic agents, there is still much room for improvement concerning the discovery of these macromolecules. Here, we present a new synthetic cell-based strategy that takes advantage of eukaryotic cell biology to produce highly diverse antibody libraries and, simultaneously, link them to a high-throughput selection mechanism, replicating B cell diversification mechanisms. The interference of site-specific recognition by CRISPR/Cas9 with error-prone DNA repair mechanisms was explored for the generation of diversity, in a cell population containing a gene for a light chain antibody fragment.

Champ4life Study Protocol: A One-Year Randomized Controlled Trial of a Lifestyle Intervention for Inactive Former Elite Athletes with Overweight/Obesity.

Preventive and educational programs directed to former elite athletes in the areas of healthy living are required. This is particularly relevant as obesity and health-related problems are observed in retired athletes, especially in those whose current levels of physical activity are below the recommendations. During their sports career, elite athletes are supported by a multidisciplinary team; upon retirement, no support is provided for the transition to a different lifestyle.

Are predictive equations a valid method of assessing the resting metabolic rate of overweight or obese former athletes?

Athletes retiring from their sports career, an understudied population, are susceptible to become overweight/obese because of decreased energy expenditure not followed by a reduction in energy intake. Therefore, their energy requirements, through resting metabolic rate (RMR), should be accurately addressed for weight management purposes. This study aimed to determine the validity of predictive equations (PEq) for RMR estimation using indirect calorimetry as the reference method in a sample of overweight/obese former athletes.

Inhibition of HIV replication through siRNA carried by CXCR4-targeted chimeric nanobody.

Small interfering RNA (siRNA) application in therapy still faces a major challenge with the lack of an efficient and specific delivery system. Current vehicles are often responsible for poor efficacy, safety concerns, and burden costs of siRNA-based therapeutics. Here, we describe a novel strategy for targeted delivery of siRNA molecules to inhibit human immunodeficiency virus (HIV) infection.

10-MDP Based Dental Adhesives: Adhesive Interface Characterization and Adhesive Stability-A Systematic Review.

The incorporation of functional monomers in dental adhesive systems promotes chemical interaction with dental substrates, resulting in higher adhesion forces when compared to micromechanical adhesion only. The 10-MDP monomer, whose chemical structure allows for a polar behavior which is favorable to adhesion, also promotes the protection of collagen fibers through the formation of MDP-calcium salts. This systematic review aimed to characterize the interface created by 10-MDP containing adhesive systems through an evaluation of the following parameters: Formation of nano-layered structures, capacity to produce an acid-base resistant zone, and adhesion stability.

Biodentine for Furcation Perforation Repair: An Animal Study with Histological, Radiographic and Micro-Computed Tomographic Assessment.

Introduction: Biodentine has been scarcely studied as a furcation perforation (FP) repair material, mostly by methodologies. This animal study aimed to compare the histological responses, radiographic, and micro-computed tomographic (micro-CT) outcomes after FP repair with Biodentine or ProRoot MTA (MTA) in dogs' teeth.

Methods And Materials: Fifty teeth from five dogs were divided into 4 groups: MTA (=20, FP repaired with ProRoot MTA), BDT (=20, FP repaired with Biodentine), PC (=5, positive control, FP without repair) and NC (=5, negative control, without perforation).

Dental Dam Application for Endodontics in Dogs-A Novel Clamp Kit.

Although rubber dams are widely used in endodontic treatment in humans with well-known advantages, their use in veterinary medicine is uncommon. The use of a dental rubber dam provides better control of cross-infection, prevents soft tissue contact of chemicals, and improves treatment efficiency. The purpose of this article is to describe a new line of clamps specifically designed for dogs and to review the procedure and materials used for isolating the operative field in endodontic patients.

Animal models used in furcation perforation studies: A systematic review and comprehensive synthesis of model characteristics.

In order to systematise furcation perforation (FP) experimental animal models described in the literature and to determine whether there is evidence that a model is superior to others, MEDLINE/PubMed, SciELO and Cochrane Library were searched to find studies which used animal models of FP. Data were gathered concerning model characteristics, chosen groups, sample numbers, type of outcomes, journal and main area studied. Twenty-five articles were included in this systematic review, four conducted in rodent models, three in non-human primates and 18 in dogs.

Nonsurgical endodontic retreatment of fused teeth with transposition: a case report.

Tooth transposition is a disorder in which a permanent tooth develops and erupts in the normal position of another permanent tooth. Fusion and gemination are developmental disturbances presenting as the union of teeth. This article reports the nonsurgical retreatment of a very rare case of fused teeth with transposition.

Antibacterial activity of Pinus elliottii and its major compound, dehydroabietic acid, against multidrug-resistant strains.

Antibiotic-resistant bacteria have emerged from the widespread use of antibiotics worldwide and have prompted the search for new sources of antimicrobial substances. Pinus spp. contain several bioactive compounds consisting mainly of terpenes, terpenoids and some other aromatic and aliphatic constituents.