Metabolomic alterations and oxidative stress are associated with environmental pollution in Procambarus clarkii.

Soils contaminated by toxic metallic elements from agricultural activities raise grave concern about their potential risk to human health through direct intake, bioaccumulation through the food chain, and their impacts on ecological systems. We have measured here the lipid and protein oxidation status and used metabolomic methodologies to identify and characterize the changes caused by metal pollution exposure in the digestive glands and gills of Procambarus clarkii, the red swamp crayfish. Specimens captured at two sites with intensive agriculture practices using diverse types of agrochemicals, located in the borders of Doñana Natural Park, were compared to ones caught in the core of the Park, a proven non-polluted place.

2D-DIGE as a proteomic biomarker discovery tool in environmental studies with Procambarus clarkii.

Unlabelled: A 2D-DIGE/MS approach was used to assess protein abundance differences in the red swamp crayfish Procambarus clarkii from polluted aquatic ecosystems of Doñana National Park and surrounding areas with different pollution loads. Procambarus clarkii accumulated metals in the digestive glands and gills reflecting sediment concentrations. We first stated that, probably related to elements accumulation, pollution increased oxidative damage in P.

Microalgae as a safe food source for animals: nutritional characteristics of the acidophilic microalga .

Background: Edible microalgae are marine or fresh water mesophilic species. Although the harvesting of microalgae offers an abundance of opportunities to the food and pharmaceutical industries, the possibility to use extremophilic microalgae as a food source for animals is not well-documented.

Objective: We studied the effects of dietary supplementation of a powdered form of the acidophilic microalga on growth and health parameters of laboratory rats.

Environmental metabolomics: Biological markers for metal toxicity.

Environmental metabolomics is an emerging field referred to the application of metabolomics to characterize the interactions of living organisms with their environment. In this sense, the importance of monitoring the effects of toxic metals on living organisms has increased as a consequence of natural changes and anthropogenic activities that have led to an increase of toxic metal levels in terrestrial and aquatic ecosystems. For this purpose, the use of metabolomics based on MS to study metal toxicity is gaining importance in recent years.

Use of elemental and molecular-mass spectrometry to assess the toxicological effects of inorganic mercury in the mouse Mus musculus.

The biochemical response of mice (Mus musculus) to acute subcutaneous inorganic-mercury exposure was assessed over a 14-day period by analyzing cytosolic extracts of the liver, the kidneys, and blood plasma. Integrated metallomic and metabolomic approaches using elemental and molecular-mass spectrometry were used to obtain comprehensive insight into the toxicological effects of mercury regarding its distribution and possible perturbation of metabolic pathways. The metallomic approach involved the use of size-exclusion chromatography (SEC) coupled with multiaffinity chromatography inductively coupled plasma-mass spectrometry (ICP-MS) and isotopic-dilution analysis.

Omics technologies and their applications to evaluate metal toxicity in mice M. spretus as a bioindicator.

Unlabelled: Metals are important components of living organisms since many biological functions critically depend on their interaction with some metal in the cell. However, human activities have increased toxic metal levels in the terrestrial and aquatic ecosystems affecting living organisms. The impact of metals on cellular metabolism and global homeostasis has been traditionally assessed in free-living organisms by using conventional biomarkers; however, to obtain a global vision of metal toxicity mechanisms and the responses that metals elicit in the organisms, new analytical methodologies are needed.

A combination of metallomics and metabolomics studies to evaluate the effects of metal interactions in mammals. Application to Mus musculus mice under arsenic/cadmium exposure.

Unlabelled: Arsenic and cadmium are toxic metals of environmental significance with harmful effects on man. To study the toxicological and biochemical effects of arsenic/cadmium in mammals a combined metallomic and metabolomic approach has been developed, complemented with the measurement of biochemical parameters in blood and histopathological evaluation of liver injury in mice Mus musculus under exposure to both xenobiotics. Size-exclusion chromatography (SEC) was combined with affinity chromatography (AF) and ICP-MS detection using species unspecific isotopic dilution analysis (SUID) to characterize the biological effects of As/Cd on selenium containing proteins in the bloodstream of exposed mice.

Heterologous microarray analysis of transcriptome alterations in Mus spretus mice living in an industrial settlement.

This work demonstrates the successful application of a commercial oligonucleotide microarray containing Mus musculus whole-genome probes to assess the biological effects of an industrial settlement on inhabitant Mus spretus mice. The transcriptomes of animals in the industrial settlement contrasted with those of specimens collected from a nearby protected ecosystem. Proteins encoded by the differentially expressed genes were broadly categorized into six main functional classes.