A novel replication initiation region encoded in a widespread Acinetobacter plasmid lineage carrying a blaNDM-1 gene.

The blaNDM-1 gene and its variants encode metallo-beta-lactamases that confer resistance to almost all beta-lactam antibiotics. Genes encoding blaNDM-1 and its variants can be found in several Acinetobacter species, and they are usually linked to two different plasmid clades. The plasmids in one of these clades contain a gene encoding a Rep protein of the Rep_3 superfamily.

A bioinformatic approach to identify confirmed and probable CRISPR-Cas systems in the - complex genomes.

Introduction: The complex, or Acb complex, consists of six species: , and . is the most clinically significant of these species and is frequently related to healthcare-associated infections (HCAIs). Clustered regularly interspaced short palindromic repeat (CRISPR) arrays and associated genes () constitute bacterial adaptive immune systems and function as variable genetic elements.

Amphibian skin bacteria display antifungal activity and induce plant defense mechanisms against .

is the causal agent of gray mold, which affects a wide variety of plant species. Chemical agents have been used to prevent the disease caused by this pathogenic fungus. However, their toxicity and reduced efficacy have encouraged the development of new biological control alternatives.

Bacteria from the skin of amphibians promote growth of Arabidopsis thaliana and Solanum lycopersicum by modifying hormone-related transcriptome response.

Plants and microorganisms establish beneficial associations that can improve their development and growth. Recently, it has been demonstrated that bacteria isolated from the skin of amphibians can contribute to plant growth and defense. However, the molecular mechanisms involved in the beneficial effect for the host are still unclear.

Draft genomes of four isolates retrieved from the elaboration process of henequen () mezcal.

We report the draft genomes of four isolates obtained from the elaboration process of henequen () mezcal, a Mexican alcoholic beverage. The average nucleotide identity analysis revealed that isolates derived from agave plants are distinct from those from other environments, including agave fermentations.

Proposal for sp. nov. to accommodate organisms of human clinical origin previously classified as genomic species 16.

In 1989, Bouvet and Jeanjean delineated five proteolytic genomic species (GS) of , each with two to four human isolates. Three were later validly named, whereas the remaining two (GS15 and GS16) have been awaiting nomenclatural clarification. Here we present the results of the genus-wide taxonomic study of 13 human strains classified as GS16 (=10) or GS15 (=3).

Inhibitory to non-inhibitory evolution of the subunit of the FF-ATPase of and -proteobacteria as related to mitochondrial endosymbiosis.

The subunit is a potent inhibitor of the FF-ATPase of (PdFF-ATPase) and related -proteobacteria different from the other two canonical inhibitors of bacterial () and mitochondrial (IF) FF-ATPases. mimics mitochondrial IF in its inhibitory N-terminus, blocking the PdFF-ATPase activity as a unidirectional pawl-ratchet and allowing the PdFF-ATP synthase turnover. is essential for the respiratory growth of , as we showed by a knockout.

Comparative Genomics of Strains Isolated from Different Ecological Niches.

The genome can change to adapt to different ecological niches. We compared four genomes from a Mexican hospital and 59 genomes from GenBank from different niches, such as urine, sputum, and environmental. The ST analysis showed that high-risk STs (ST235, ST773, and ST27) were present in the genomes of the three niches from GenBank, and the STs of Mexican genomes (ST167, ST2731, and ST549) differed from the GenBank genomes.

Recombinant BL21 with LngA Variants from ETEC E9034A Promotes Adherence to HT-29 Cells.

Unlabelled: The CS21 pilus produced by enterotoxigenic (ETEC) is involved in adherence to HT-29 intestinal cells. The CS21 pilus assembles proteins encoded by 14 genes clustered into the operon.

Aim: This study aimed to determine whether .

A Plasmid Carrying in Belonging to a Novel Resistance Plasmid Family.

and are the most detected plasmid-encoded carbapenemase genes in . Previous studies have reported plasmid sequences carrying variants, except . In this study, we aimed to characterize a plasmid carrying in a strain isolated from a Mexican hospital.

New Alphaproteobacteria Thrive in the Depths of the Ocean with Oxygen Gradient.

We survey here the Alphaproteobacteria, a large class encompassing physiologically diverse bacteria which are divided in several orders established since 2007. Currently, there is considerable uncertainty regarding the classification of an increasing number of marine metagenome-assembled genomes (MAGs) that remain poorly defined in their taxonomic position within Alphaproteobacteria. The traditional classification of NCBI taxonomy is increasingly complemented by the Genome Taxonomy Database (GTDB), but the two taxonomies differ considerably in the classification of several Alphaproteobacteria, especially from ocean metagenomes.

Replication initiator proteins of Acinetobacter baumannii plasmids: An update note.

The bioinformatic analysis that we made of 492 Acinetobacter baumannii plasmid sequences identified 418 genes encoding Replication Initiator (Rep) proteins that fell into at least fourteen groups according to the protein domains that they contained. The most abundant group of Rep proteins contained a Rep_3 superfamily domain, followed by Rep proteins containing Replicase/PriCT_1 superfamily domains, and then by Reps possessing only an HTH_MerR-SF superfamily domain. The remaining eleven groups contain only a few members.

Complete Genome Sequence of Acinetobacter junii Strain INC8271, Isolated from a Patient with Metastatic Cancer and Bacteremia.

Acinetobacter junii INC8271 was isolated from a cancer patient with polymicrobial bacteremia after biliary stent placement. The complete genome sequence consisted of a chromosome of 3,530,883 bp (GC content, 38.56%) with 3,377 genes, including those encoding 74 tRNAs and 18 rRNAs, and two intact prophage sequences.

A novel vieuvirus from multidrug-resistant Acinetobacter baumannii.

Bacteriophages are considered the most abundant biological entities on earth, and they are able to modulate the populations of their bacterial hosts. Although the potential of bacteriophages has been accepted as an alternative strategy to combat multidrug-resistant pathogenic bacteria, there still exists a considerable knowledge gap regarding their genetic diversity, which hinders their use as antimicrobial agents. In this study, we undertook a genomic and phylogenetic characterization of the phage Ab11510-phi, which was isolated from a multidrug-resistant Acinetobacter baumannii strain (Ab11510).

The mitogenome of , a desiccation-tolerant moss.

Bryophytes are the earliest plant group on Earth. They are a fundamental component of many ecosystems around the World. Some of their main roles are related to soil development, water retention, and biogeochemical cycling.

Molecular Epidemiology of - Complex Isolated From Children at the Hospital Infantil de México Federico Gómez.

The - () complex is regarded as a group of phenotypically indistinguishable opportunistic pathogens responsible for mainly causing hospital-acquired pneumonia and bacteremia. The aim of this study was to determine the frequency of isolation of the species that constitute the complex, as well as their susceptibility to antibiotics, and their distribution at the Hospital Infantil de Mexico Federico Gomez (HIMFG). A total of 88 strains previously identified by Vitek 2®, 40 as and 48 as complex were isolated from 52 children from 07, January 2015 to 28, September 2017.

High mortality in an outbreak of multidrug resistant Acinetobacter baumannii infection introduced to an oncological hospital by a patient transferred from a general hospital.

Objective: To describe the clinical features, outcomes, and molecular epidemiology of an outbreak of multidrug resistant (MDR) A. baumannii.

Methods: We performed a retrospective analysis of all MDR A.

Chromosome Architecture and Gene Content of the Emergent Pathogen .

is a Gammaproteobacterium that has been involved in serious diseases frequently linked to the nosocomial environment. Most of the strains causing such infections are sensitive to a wide variety of antibiotics, but recent reports indicate that this pathogen is acquiring very efficiently carbapenem-resistance determinants like the NDM-1 gene, all over the world. With this work we contribute with a collection set of 31 newly sequenced nosocomial isolates.

Structure and Evolution of Plasmids.

is an emergent bacterial pathogen that provokes many types of infections in hospitals around the world. The genome of this organism consists of a chromosome and plasmids. These plasmids vary over a wide size range and many of them have been linked to the acquisition of antibiotic-resistance genes.

Mucosal Microbiome Profiles Polygenic Irritable Bowel Syndrome in Mestizo Individuals.

Irritable bowel syndrome (IBS) is the most frequent functional gastrointestinal disorder, worldwide, with a high prevalence among Mestizo Latin Americans. Because several inflammatory disorders appear to affect this population, a further understanding of host genomic background variants, in conjunction with colonic mucosa dysbiosis, is necessary to determine IBS physiopathology and the effects of environmental pressures. Using a simple polygenic model, host single nucleotide polymorphisms (SNPs) and the taxonomic compositions of microbiota were compared between IBS patients and healthy subjects.