Publications by authors named "Mieko Yamada"
Application of heavy-ion-beam irradiation to breeding large rotifer.
Biosci Biotechnol Biochem
February 2021
Article Synopsis
- In larviculture, rotifers are crucial as an initial food source for fish larvae, but their size and density must be managed properly to prevent mortality and developmental issues.
- The study applied carbon and argon heavy-ion-beam irradiation to create rotifer mutants with larger sizes, assessing factors like lethality and growth rates.
- Three promising rotifer mutants, TYC78, TYC176, and TYA41, demonstrated larger sizes and active population growth, indicating their potential as a solution for improving live feed in fish larviculture.
Heavy-ion irradiation is a powerful mutagen that possesses high linear energy transfer (LET). Several studies have indicated that the value of LET affects DNA lesion formation in several ways, including the efficiency and the density of double-stranded break induction along the particle path. We assumed that the mutation type can be altered by selecting an appropriate LET value.
View Article and Find Full Text PDFDetection of mutations at the whole-genome level is now possible by the use of high-throughput sequencing. However, determining mutations is a time-consuming process due to the number of false positives provided by mutation-detecting programs. AMAP (automated mutation analysis pipeline) was developed to overcome this issue.
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