Publications by authors named "Mie Fukuda"
Chinese hamster ovary (CHO) cells are widely used for the industrial production of therapeutic monoclonal antibodies (mAbs). To meet the increasing market demands, high productivity, and quality are required in cell culture. One of the critical attributes of mAbs, from a safety perspective, is mAb fragmentation.
View Article and Find Full Text PDFArticle Synopsis
- Interchain disulfide bonds in monoclonal antibodies can be reduced during large-scale production using CHO cells, leading to lower product yield and purity.
- The reduction susceptibility varies by cell line, and there hasn't been an effective method to screen for reduction-resistant CHO cells until now.
- The new screening method can test up to 48 cell lines simultaneously and reveals that reduction-susceptible lines have higher NADPH and NADP levels, suggesting that this method could help improve mAb production.