Oral administration of a new copper (I) complex with coumarin as ligand: modulation of the immune response and the composition of the intestinal microbiota in .

[Cu(NN)]ClO is a copper (I) complex, where NN is an imine ligand 6-((quinolin-2-ylmethylene) amino)-2H-chromen-2-one obtained by derivatization of natural compound coumarin, developed for the treatment of infectious diseases that affect salmonids. In previous research, we showed that the Cu(I) coordination complex possesses antibacterial activity against , providing protection against this pathogen in rainbow trout during challenge assays (with an RPS of 50%). In the present study, the effects of administering [Cu(NN)]ClO to over a 60-days period were evaluated with regard to systemic immune response and its potential to alter intestinal microbiota composition.

[Cu(NN)]ClO, a Copper (I) Complex as an Antimicrobial Agent for the Treatment of Piscirickettsiosis in Atlantic Salmon.

is the pathogen that most affects the salmon industry in Chile. Large quantities of antibiotics have been used to control it. In search of alternatives, we have developed [Cu(NN)]ClO where NN = 6-((quinolin-2-ylmethylene)amino)-2H-chromen-2-one.

Importance of Probiotics in Fish Aquaculture: Towards the Identification and Design of Novel Probiotics.

Aquaculture is a growing industry worldwide, but it faces challenges related to animal health. These challenges include infections by parasites, bacteria, and viral pathogens. These harmful pathogens have devastating effects on the industry, despite efforts to control them through vaccination and antimicrobial treatments.

Analysis of microbiota-host communication mediated by butyrate in Atlantic salmon.

Butyrate is a microbiota-produced metabolite, sensed by host short-chain fatty acid receptors FFAR2 (Gpr43), FFAR3 (Gpr41), HCAR2 (Gpr109A), and Histone deacetylase (HDAC) that promotes microbiota-host crosstalk. Butyrate influences energy uptake, developmental and immune response in mammals. This microbial metabolite is produced by around 79 anaerobic genera present in the mammalian gut, yet little is known about the role of butyrate in the host-microbiota interaction in salmonid fish.

Protective Effect of [Cu(NN)](ClO) Complex in Rainbow Trout Challenged against .

Previously, we reported an in vitro evaluation regarding antibacterial effects against by a new Cu (I) complex, [Cu(NN)](ClO). This study presents the results of an in vivo evaluation of [Cu(NN)](ClO) added as a dietary supplement against in rainbow trout. The results showed that the administration of [Cu(NN)](ClO) at 29 and 58 µg/g of fish for 15 days does not affect the growth of rainbow trout.

In Vitro Effect of Copper (I) Complex [Cu(NN)](ClO) on BB170 Biofilm Formation.

Biofilm formation in pathogenic bacteria is an important factor of resistance to antimicrobial treatments, allowing them to survive for a long time in their hosts. In the search for new antibiofilm agents, in this work we report the activity of a copper (I) complex, [Cu(NN)]ClO, synthesized with Cu (I) and NN1, an imine ligand 6-((quinolin-2-ylmethylene)amino)-2H-chromen-2-one, a derivate of natural compound coumarin. The antibacterial and antibiofilm capacity was evaluated in BB170 used as model bacteria.

Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response and .

In salmon farming, viruses are responsible for outbreaks that produce significant economic losses for which there is a lack of control tools other than vaccines. Type I interferon has been successfully used for treating some chronic viral infections in humans. However, its application in salmonids depends on the proper design of a vehicle that allows its massive administration, ideally orally.

Oral Administration of Producing Interferon Type II, Enhances the Immune Response Against Bacterial Pathogens in Rainbow Trout.

Lactic acid bacteria are a powerful vehicle for releasing of cytokines and immunostimulant peptides at the gastrointestinal level after oral administration. However, its therapeutic application against pathogens that affect rainbow trout and Atlantic salmon has been little explored. Type II interferon in Atlantic salmon activates the antiviral response, protecting against viral infection, but its role against bacterial infection has not been tested In this work, through the design of a recombinant lactic acid bacterium capable of producing Interferon gamma from Atlantic salmon, we explore its role against bacterial infection and the ability to stimulate systemic immune response after oral administration of the recombinant probiotic.

Microbiota Modulates the Immunomodulatory Effects of Filifolinone on Atlantic Salmon.

Filifolinone is an aromatic geranyl derivative, a natural compound isolated from , which has immunomodulatory effects on Atlantic salmon, upregulating cytokines involved in Th1-type responses through a mechanism that remains unknown. In this work, we determined whether the immunomodulatory effects of filifolinone depend on the host microbiotic composition. We evaluated the effect of filifolinone on immune genes and intestinal microbiotic composition of normal fish and fish previously treated with bacitracin/neomycin.

New Copper(I) Complex with a Coumarin as Ligand with Antibacterial Activity against .

A new copper (I) complex, [Cu(NN)](ClO) was synthesized, where NN was a imine ligand 6-((quinolin-2-ylmethylene)amino)-2H-chromen-2-one obtained by a derivatization of natural compound coumarin. The structural characterization in solution was done by NMR techniques, UV-Vis and cyclic voltammetry. The potential antibacterial effect of [Cu(NN)](ClO) was assessed for isolated 10094.