Stability of Terpenoid-Derived Secondary Ozonides in Aqueous Organic Media.

1,2,4-Trioxolanes, known as secondary ozonides (SOZs), are key products of ozonolysis of biogenic terpenoids. Functionalized terpenoid-derived SOZs are readily taken up into atmospheric aerosols; however, their condensed-phase fates remain unknown. Here, we report the results of a time-dependent mass spectrometric investigation into the liquid-phase fates of C and C SOZs synthesized by ozonolysis of a C monoterpene alcohol (α-terpineol) in water:acetone (1:1 = vol:vol) mixtures.

Novel mutations of neurofibromatosis type 1 gene in small cell lung cancers.

Purpose: Mutations in the GTPase-activating protein-related domain (GRD) of the von Recklinghausen neurofibromatosis type 1 (NF1) gene have been reported in several tumors that were not previously associated with NF1. We analyzed 37 cases of lung cancer, including 9 cases of small cell carcinoma, to detect mutations in this domain.

Methods: DNA was extracted from the tumors, and single-strand conformation polymorphism and direct sequencing were used to detect mutations.