Daily low-intensity pulsed ultrasound-mediated osteogenic differentiation in rat osteoblasts.

There were few studies investigating the effects of the mechanical stimulation provided by daily low-intensity pulsed ultrasound (LIPUS) treatment. LIPUS is known to accelerate bone mineralization and regeneration; however, the precise cellular mechanism is unclear.Our purpose was to determine how daily LIPUS treatment affected cell viability, alkaline phosphatase activity, osteogenesis-related gene expression, and mineralized nodule formation in osteoblasts.