Publications by authors named "Michiharu Nakano"

This study was designed to perform an association analysis and identify SNP markers associated with production traits of Japanese quail using restriction-site-associated DNA sequencing. Weekly body weight data from 805 quail were collected from hatching to 16 weeks of age. A total number of 3990 eggs obtained from 399 female quail were used to assess egg quality traits.

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White rust caused by is one of the most serious diseases of chrysanthemum ( × ). In this study, we report the DNA markers associated with resistance against via a simple approach using the genome of a wild diploid relative, . First, we identified the important region of the genome in the resistant cultivar "Ariesu" via a genome-wide association study.

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In this study, we analyzed the complete sequence of the chloroplast genome of Matsum. et Koidz., 1910, a diploid disciform capitula species of endemic to Japan, formerly classified as (Matsum.

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Chrysanthemums are one of the most industrially important cut flowers worldwide. However, their segmental allopolyploidy and self-incompatibility have prevented the application of genetic analysis and modern breeding strategies. We thus developed a model strain, Gojo-0 (Chrysanthemum seticuspe), which is a diploid and self-compatible pure line.

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Root hair cells form the primary interface of plants with the soil environment, playing key roles in nutrient uptake and plant defense. In legumes, they are typically the first cells to become infected by nitrogen-fixing soil bacteria during root nodule symbiosis. Here, we report a role for the CELLULOSE SYNTHASE-LIKE D1 (CSLD1) gene in root hair development in the legume species Lotus japonicus.

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This study aimed to identify quantitative trait loci (QTLs) for growth-related traits by constructing a genetic linkage map based on single nucleotide polymorphism (SNP) markers in Japanese quail. A QTL mapping population of 277 F birds was obtained from an intercross between a male of a large-sized strain and three females of a normal-sized strain. Body weight (BW) was measured weekly from hatching to 16 weeks of age.

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This research was conducted to identify quantitative trait loci (QTL) associated with egg-related traits by constructing a genetic linkage map based on single nucleotide polymorphism (SNP) markers using restriction-site associated DNA sequencing (RAD-seq) in Japanese quail. A total of 138 F females were produced by full-sib mating of F birds derived from an intercross between a male of the large-sized strain with three females of the normal-sized strain. Eggs were investigated at two different stages: the beginning stage of egg-laying and at 12 weeks of age (second stage).

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Sorghum [Sorghum bicolor (L.) Moench] grown locally by Japanese farmers is generically termed Takakibi, although its genetic diversity compared with geographically distant varieties or even within Takakibi lines remains unclear. To explore the genomic diversity and genetic traits controlling biomass and other physiological traits in Takakibi, we focused on a landrace, NOG, in this study.

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Article Synopsis
  • * Chrysanthemum morifolium faces challenges in genetic analysis and breeding due to its autohexaploid nature and high genome heterogeneity, prompting the development of a more manageable model strain called Gojo-0 from the diploid C. seticuspe.
  • * Gojo-0 is advantageous for genetic studies and breeding as it allows for the isolation of mutants and easier genome analysis, enabling further research and genetic improvements in chrysanthemum cultivars.
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Cultivated chrysanthemum (Chrysanthemum morifolium Ramat.) is one of the most economically important ornamental crops grown worldwide. It has a complex hexaploid genome (2n = 6x = 54) and large genome size.

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Background: Somatic embryogenesis in nucellar tissues is widely recognized to induce polyembryony in major citrus varieties such as sweet oranges, satsuma mandarins and lemons. This capability for apomixis is attractive in agricultural production systems using hybrid seeds, and many studies have been performed to elucidate the molecular mechanisms of various types of apomixis. To identify the gene responsible for somatic embryogenesis in citrus, a custom oligo-DNA microarray including predicted genes in the citrus polyembryonic locus was used to compare the expression profiles in reproductive tissues between monoembryonic and polyembryonic varieties.

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strain MO-1, which possesses a high ability to oxidize Mn(II), has been isolated from oligotrophic pond sediment. The draft genome sequence consists of 6,252,942 bp and has a G+C content of 63.4%.

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Chlorophyll is an essential molecule for acquiring light energy during photosynthesis. Mutations that result in chlorophyll retention during leaf senescence are called 'stay-green' mutants. One of the several types of stay-green mutants, Type E, accumulates high levels of chlorophyll in the pre-senescent leaves, resulting in delayed yellowing.

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Article Synopsis
  • Chlorophyll degradation is crucial for leaf senescence and involves the breakdown of chlorophyll-binding proteins, but the full regulation of the process is still not completely understood.
  • Green cotyledon mutants in legumes are unique because they show impaired chlorophyll degradation during leaf aging and have a maternally inherited soybean gene that affects this process.
  • Further research revealed that a specific 5-bp insertion in the gene affects photosystem II, leading to reduced chlorophyll degradation activity in mutant plants, suggesting that this gene uniquely connects photosynthesis and chlorophyll breakdown during leaf senescence and seed maturation.
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To explore the transcription factors associated with carotenoid metabolism in citrus fruit, one transcription factor (CubHLH1) was selected through microarray screening in Satsuma mandarin (Citrus unshiu Marc.) fruit, which was treated with exogenous ethylene or gibberellin (GA), accelerating or retarding carotenoid accumulation in peel, respectively. The amino acid sequence of CubHLH1 has homology to Arabidopsis activation-tagged bri1 suppressor 1 (ATBS1) interacting factor (AIF), which is functionally characterized as a negative regulator of the brassinolide (BR) signalling pathway.

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Article Synopsis
  • Recent whole-genome sequencing of soybean uncovered significant duplications that contributed to its unique genome structure, indicating a complex evolutionary history.
  • A natural green-cotyledon mutant, Tenshin-daiseitou, was found to have a stay-green phenotype due to mutations in GmSGR1 and GmSGR2, which play a role in chlorophyll degradation during leaf senescence.
  • Analysis revealed four genomic regions related to chlorophyll degradation genes, demonstrating a connection between whole-genome duplications and the evolution of soybean traits.
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The fruit of melting-flesh peach (Prunus persica L. Batsch) cultivars produce high levels of ethylene caused by high expression of PpACS1 (an isogene of 1-aminocyclopropane-1-carboxylic acid synthase), resulting in rapid fruit softening at the late-ripening stage. In contrast, the fruit of stony hard peach cultivars do not soften and produce little ethylene due to low expression of PpACS1.

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Polyembryony, in which multiple somatic nucellar cell-derived embryos develop in addition to the zygotic embryo in a seed, is common in the genus Citrus. Previous genetic studies indicated polyembryony is mainly determined by a single locus, but the underlying molecular mechanism is still unclear. As a step towards identification and characterization of the gene or genes responsible for nucellar embryogenesis in Citrus, haplotype-specific physical maps around the polyembryony locus were constructed.

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