Eomes-dependent mitochondrial regulation promotes survival of pathogenic CD4+ T cells during inflammation.

The mechanisms whereby Eomes controls tissue accumulation of T cells and strengthens inflammation remain ill-defined. Here, we show that Eomes deletion in antigen-specific CD4+ T cells is sufficient to protect against central nervous system (CNS) inflammation. While Eomes is dispensable for the initial priming of CD4+ T cells, it is required for long-term maintenance of CNS-infiltrating CD4+ T cells.

Serotonin reduction in post-acute sequelae of viral infection.

Post-acute sequelae of COVID-19 (PASC, "Long COVID") pose a significant global health challenge. The pathophysiology is unknown, and no effective treatments have been found to date. Several hypotheses have been formulated to explain the etiology of PASC, including viral persistence, chronic inflammation, hypercoagulability, and autonomic dysfunction.

Quantitative control of Ets1 dosage by a multi-enhancer hub promotes Th1 cell differentiation and protects from allergic inflammation.

Multi-enhancer hubs are spatial clusters of enhancers present across numerous developmental programs. Here, we studied the functional relevance of these three-dimensional structures in T cell biology. Mathematical modeling identified a highly connected multi-enhancer hub at the Ets1 locus, comprising a noncoding regulatory element that was a hotspot for sequence variation associated with allergic disease in humans.

175 W average power from a single-core rod fiber-based chirped-pulse-amplification system.

We report on a fiber-based chirped-pulse-amplification laser system with bulk transmission grating compression to a pulse duration of 357 fs, average power of 175 W, and pulse energy of 233 . The compressed pulse train has a beam quality factor M of 1.21.

The miR-181 family regulates colonic inflammation through its activity in the intestinal epithelium.

The intestinal epithelium is a key physical interface that integrates dietary and microbial signals to regulate nutrient uptake and mucosal immune cell function. The transcriptional programs that regulate intestinal epithelial cell (IEC) quiescence, proliferation, and differentiation have been well characterized. However, how gene expression networks critical for IECs are posttranscriptionally regulated during homeostasis or inflammatory disease remains poorly understood.

Multiscale 3D genome organization underlies ILC2 ontogenesis and allergic airway inflammation.

Innate lymphoid cells (ILCs) are well-characterized immune cells that play key roles in host defense and tissue homeostasis. Yet, how the three-dimensional (3D) genome organization underlies the development and functions of ILCs is unknown. Herein, we carried out an integrative analysis of the 3D genome structure, chromatin accessibility and gene expression in mature ILCs.

Observation of dynamical eigenmodes induced by the moving refractive index grating of TMI in a rod amplifier.

We report a novel, to the best of our knowledge, analysis of high power rod fiber amplifiers by monitoring the cross-polarization of the output. Spatially and temporally resolved imaging of co- and cross-polarizations at high power amplification reveals dynamic eigenmode behavior of the rod fiber. The dynamic of the eigenmodes is caused by the moving refractive index grating written by the modal interference pattern of transverse mode instability and is the first direct observation of this refractive index grating, to our knowledge.

A new perspective on mesenchymal-immune interactions in adipose tissue.

The mammalian immune system has crucial homeostatic functions in different adipose depots. However, white adipose tissue (WAT) inflammation is a hallmark of obesity and can contribute to type 2 diabetes mellitus (T2DM). Recently, mesenchymal cells were identified as highly heterogenous populations displaying specialized immune functions in immune cell migration, activation, survival, and overall lymphoid tissue organization in several tissues.

Ontogeny and heterogeneity of innate lymphoid cells and the noncoding genome.

Since their discovery a decade ago, it has become evident that innate lymphoid cells (ILCs) play critical roles in protective immune responses against intracellular and extracellular pathogens but are also central regulators of epithelial barrier integrity and tissue homeostasis. ILCs populate almost every tissue in mammalian organisms; therefore, not surprisingly, dysregulation of their functions contributes to the development and progression of multiple inflammatory and metabolic diseases. Our knowledge of the transcriptional programs governing the development, differentiation, and functions of the different groups of ILCs has increased dramatically in the last ten years.

Spectral broadening of ultraviolet dispersive waves in gas-filled hollow-core fiber using pump pulse modulation.

UV supercontinuum laser sources based on resonant dispersive wave (RDW) generation in gas-filled hollow-core (HC) fibers offer an attractive architecture for numerous applications. However, the narrow UV spectral peak inherent to RDW generation limits the suitability for applications that require broad spectral coverage within the UV region such as spectroscopic scatterometry. In this Letter, we demonstrate how the UV spectrum can be shaped by modulating the peak power of the pump pulses driving the RDW generation, thereby creating a broadened and flattened UV spectrum.

Low-noise tunable deep-ultraviolet supercontinuum laser.

The realization of a table-top tunable deep-ultraviolet (UV) laser source with excellent noise properties would significantly benefit the scientific community, particularly within imaging and spectroscopic applications, where source noise has a crucial role. Here we provide a thorough characterization of the pulse-to-pulse relative intensity noise (RIN) of such a deep-UV source based on an argon (Ar)-filled anti-resonant hollow-core (AR HC) fiber. Suitable pump pulses are produced using a compact commercially available laser centered at 1030 nm with a pulse duration of 400 fs, followed by a nonlinear compression stage that generates pulses with 30 fs duration, 24.

Experimental investigations of seeding mechanisms of TMI in rod fiber amplifier using spatially and temporally resolved imaging.

In this work we investigate transverse mode instability (TMI) in the presence of pump intensity noise and a controlled perturbation of the input coupling for a rod-type fiber amplifier using spatially and temporally resolved imaging (ST). We show that inherent pump intensity noise from the power supply can define significant peaks in the resulting TMI spectrum. ST measurements show that the TMI in the transition region consists of different orientations of LP.

Static and dynamic mode coupling in a double-pass rod-type fiber amplifier.

This Letter describes an experimental realization of a double-pass amplifier using rod-type fiber. In this device, the gain reaches 26 dB amplifying a 300 mW, 20 ps, 20 MHz seed up to 120 W, with an optical-to-optical efficiency of 50% and excellent beam quality. In addition, by design the output of the amplifier has a polarization extinction ratio of 33 dB.

Flexible cross-correlated (C) imaging method for the modal content characterization in a broad range of wavelengths.

We demonstrate a flexible cross-correlated (C) imaging method in the time domain by application of a tunable and highly flexible light source. An advantage of the flexible C method is shown by characterization of the step-index fiber (SMF28) over a broad range of wavelengths from 870nm to 1090nm and by the modal analysis of the distributed modal filtering (DMF) rod fiber within a wavelength range from 1050nm to 1090nm. Also, the influence of the spectral shape and bandwidth on the imaging trace is investigated by deliberately adjusting the input spectrum of the light source.

Foxo3 Transcription Factor Drives Pathogenic T Helper 1 Differentiation by Inducing the Expression of Eomes.

The transcription factor Foxo3 plays a crucial role in myeloid cell function but its role in lymphoid cells remains poorly defined. Here, we have shown that Foxo3 expression was increased after T cell receptor engagement and played a specific role in the polarization of CD4 T cells toward pathogenic T helper 1 (Th1) cells producing interferon-γ (IFN-γ) and granulocyte monocyte colony stimulating factor (GM-CSF). Consequently, Foxo3-deficient mice exhibited reduced susceptibility to experimental autoimmune encephalomyelitis.

Cognitive Reserve in Dementia: Implications for Cognitive Training.

Cognitive reserve (CR) is a potential mechanism to cope with brain damage. The aim of this study was to evaluate the effect of CR on a cognitive training (CT) in a group of patients with dementia. Eighty six participants with mild to moderate dementia were identified by their level of CR quantified by the CR Index questionnaire (CRIq) and underwent a cycle of CT.

Hollow-core fibers for high power pulse delivery.

We investigate hollow-core fibers for fiber delivery of high power ultrashort laser pulses. We use numerical techniques to design an anti-resonant hollow-core fiber having one layer of non-touching tubes to determine which structures offer the best optical properties for the delivery of high power picosecond pulses. A novel fiber with 7 tubes and a core of 30µm was fabricated and it is here described and characterized, showing remarkable low loss, low bend loss, and good mode quality.

Compact, CO-stabilized tuneable laser at 2.05 microns.

We demonstrate a compact fibre-based laser system at 2.05 microns stabilized to a CO transition using frequency modulation spectroscopy of a gas-filled hollow-core fibre. The laser exhibits an absolute frequency accuracy of 5 MHz, a frequency stability noise floor of better than 7 kHz or 5 × 10 and is tunable within ±200 MHz from the molecular resonance frequency while retaining roughly this stability and accuracy.

Optical frequency standard using acetylene-filled hollow-core photonic crystal fibers.

Gas-filled hollow-core photonic crystal fibers are used to stabilize a fiber laser to the 13C2H2 P(16) (ν1+ν3) transition at 1542 nm using saturated absorption. Four hollow-core fibers with different crystal structure are compared in terms of long term lock-point repeatability and fractional frequency instability. The locked fiber laser shows a fractional frequency instability below 4 × 10(-12) for averaging time up to 10(4) s.

Cladding defects in hollow core fibers for surface mode suppression and improved birefringence.

We demonstrate a novel polarization maintaining hollow-core photonic bandgap fiber geometry that reduces the impact of surface modes on fiber transmission. The cladding structure is modified with a row of partially collapsed holes to strip away unwanted surface modes. A theoretical investigation of the surface mode stripping is presented and compared to the measured performance of four 7-cells core fibers that were drawn with different collapse ratio of the defects.

Predictors of left atrium appendage clot detection despite on-target warfarin prevention for atrial fibrillation.

Background And Purpose: The antithrombotic management of atrial fibrillation (AF) is currently based on clinical scores (CHADS(2) or CHA(2)DS(2)VASc). The prevalence of left atrium (LA) thrombi in effectively anticoagulated AF patients has been reported as being up to 7.7 %.

Variables correlated with early relapses after external electrical cardioversion of persistent atrial fibrillation.

Background: The aim of this study was to identify the clinical and echocardiographic variables possibly correlated with the early relapses of atrial fibrillation (AF) after external electrical cardioversion (EC) in a large cohort of patients with persistent AF.

Methods: Two hundred patients (117 males, 83 females, mean age 67.9 +/- 8.

Effects of verapamil and metoprolol on recovery from atrial electrical remodeling after cardioversion of long-lasting atrial fibrillation.

The aim of this prospective, randomized study was to investigate the effect of pretreatment with two different intracellular calcium-lowering drugs (verapamil and metoprolol) on recovery from atrial effective refractory period (AERP) shortening after internal electrical cardioversion (EC) of persistent atrial fibrillation (AF) in patients on amiodarone. Twenty-one patients on amiodarone for at least 30 days were referred to our hospital for internal EC of a persistent AF refractory to external EC. They were randomized to receive only amiodarone (group AMI, n=7), or amiodarone and verapamil 240 mg/day (group VER, n=7), or amiodarone and metoprolol 100 mg/day (group MET, n=7).

Determinants of left ventricular structure and mass in young subjects with sympathetic over-activity. The Tecumseh Offspring Study.

Objective: In this study, we tested the hypothesis that sympathetic over-activity may cause metabolic abnormalities and affect left ventricular (LV) structure and mass early in life.

Subjects And Setting: The study population consisted of 111 healthy adolescents and young adults living in Tecumseh, Michigan (USA).

Main Outcome Measures: Correlations of LV mass and structure with several clinical variables in relation to the activity of the sympathetic nervous system.

Right bundle branch block, intermittent ST segment elevation and inducible ventricular tachycardia in an asymptomatic patient: an unusual presentation of the Brugada syndrome?

We describe the case of an asymptomatic 54-year-old female who underwent a routine electrocardiogram (ECG) in July 1997, which showed incomplete right bundle branch block and an important ST segment elevation with a coved fashion and the inversion of T waves in leads V1-V2. Her family and personal history was free of any cardiovascular pathology. She previously underwent a routine ECG in 1991 and 1995, showing an incomplete right bundle branch block with a moderate ST segment elevation in leads V1-V2.