Synthesis, structure, photophysical and electrochemical properties of Ru(TFA)(CO)(PPh)(L) (L=2-phenylpyridine, 2--tolylpyridine) and Ru(CO)(PPhMe)(L)(L') (L= TFA, H) (L'= bipyridine, L'= 4,4'-dimethylbipyridine) relationships between ancillary ligand structure and luminescent properties.

The synthesis, structure and photophysical properties of the complexes [Ru[(CO)(TFA) (PPh)(L)] [(L = ppy = 2-phenylpyridine, (); L = 2-(p-tolyl)pyridine] (), are reported. The complexes were characterized by UV-VIS, IR and NMR and by single-crystal X-ray diffraction techniques. We also report the synthesis, structure and photophysical properties of [Ru(CO)(L)(PPhMe)(L')][PF]- [L' = bipyridine, L = TFA, (); L = H, () and L = H, L' = 4,4'-dimethlyl bipyridine ()].

Nanosecond Dynamics of Gαi1 Bound to Nucleotides or Ric-8A, a Gα Chaperone with GEF Activity.

Resistance to Inhibitors of Cholinesterase A (Ric-8A) is a 60-kDa cytosolic protein that has chaperone and guanine nucleotide exchange (GEF) activity toward heterotrimeric G protein α subunits of the i, q, and 12/13 classes, catalyzing the release of GDP from Gα and subsequent binding of GTP. In the absence of GTP or GTP analogs, and subsequent to GDP release, Gα forms a stable nucleotide-free complex with Ric-8A. In this study, time-resolved fluorescence anisotropy measurements were employed to detect local motions of Gαi1 labeled at selected sites with Alexa 488 (C5) fluorescent dye (Ax) in the GDP, GTPγS (collectively, GXP), and Ric-8A-bound states.

Luminescence Quenching and Enhancement by Adsorbed Metal Ions with Ruthenium Diimine Complexes Immobilized on Silica Polyamine Composites.

Luminescent ruthenium diimine complexes have been covalently bound to the surface of a silica polyamine composite (SPC) using peptide coupling agents. The loading of the complexes using this route is quite low (~0.01-0.

Surface-Bound Ruthenium Diimine Organometallic Complexes: Excited-State Properties.

Ruthenium complexes of the general formula [Ru(CO)(H)(L)(L')][PF] (L = 2PPh, L' = η-4,4'-dicarboxybipyridine (); L =-2PhPCHCHCOOH, L' = bipyridine (); L = PhPCHCHPPh, L' = η-5-amino-1,10-phenanthroline (); L = 2PPh, L' = η-4-carboxaldehyde-4'-methylbipyridine ()) have been shown to have longer emission lifetimes and higher quantum yields in solution compared with more symmetrical molecules such as [Ru(bpy)][Cl]. Compound is obtained as a mixture with the corresponding acetal, . These less symmetrical complexes have been covalently immobilized on the surface of silica polyamine composites, and their photophysical properties have been studied.

Photophysical studies of bioconjugated ruthenium metal-ligand complexes incorporated in phospholipid membrane bilayers.

The luminescent, mono-diimine ruthenium complexes [(H)Ru(CO)(PPh3)2(dcbpy)][PF6] (1) (dcbpy = 4,4'-dicarboxy-2,2'-bipyridyl) and [(H)Ru(CO)(dppene)(5-amino-1,10-phen)][PF6] (2) (dppene = bis(diphenylphosphino)ethylene; phen = phenanthroline) were conjugated with 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (DPPE) and with cholesterol in the case of complex 2. Using standard conjugation techniques, compound 1 gives the bis-lipid derivative [(H)Ru(CO)(PPh3)2(dcbpy-N-DPPE2)][PF6] (3), while 2 provides the monolipid conjugate [(H)Ru(CO)(dppene)(1,10-phen-5-NHC(S)-N-DPPE)][PF6] (4) and the cholesterol derivative [(H)Ru(CO)(dppene)(1,10-phen-5-NHC(O)Ocholesteryl)][PF6] (5). These compounds were characterized by spectroscopic methods, and their photophysical properties were measured in organic solvents.