Comparing the Effects of Rocaglates on Energy Metabolism and Immune Modulation on Cells of the Human Immune System.

A promising new approach to broad spectrum antiviral drugs is the inhibition of the eukaryotic translation initiation factor 4A (elF4A), a DEAD-box RNA helicase that effectively reduces the replication of several pathogenic virus types. Beside the antipathogenic effect, modulation of a host enzyme activity could also have an impact on the immune system. Therefore, we performed a comprehensive study on the influence of elF4A inhibition with natural and synthetic rocaglates on various immune cells.

Interactions between a dsDNA Oligonucleotide and Imidazolium Chloride Ionic Liquids: Effect of Alkyl Chain Length, Part I.

Ionic liquids (ILs) have become nearly ubiquitous solvents and their interactions with biomolecules has been a focus of study. Here, we used the fluorescence emission of DAPI, a groove binding fluorophore, coupled with molecular dynamics (MD) simulations to report on interactions between imidazolium chloride ([Im]) ionic liquids and a synthetic DNA oligonucleotide composed entirely of T/A bases (7(TA)) to elucidate the effects ILs on a model DNA duplex. Spectral shifts on the order of 500-1000 cm, spectral broadening (~1000 cm), and excitation and emission intensity ratio changes combine to give evidence of an increased DAPI environment heterogeneity on added IL.

Sublabral foramen and buford complex: inferior extent of the unattached or absent labrum in 50 patients.

Purpose: To determine the inferiormost extent of the anterosuperior labral variants on conventional transverse MR images.

Materials And Methods: We reviewed transverse MR images in 50 consecutive patients with a sublabral foramen or Buford complex at arthroscopy. Images were randomly mixed with those of 58 patients with either a normal labrum (n = 20) or an anterior labral tear (n = 38) at arthroscopy.