Naming CRISPR alleles: endonuclease-mediated mutation nomenclature across species.

The widespread use of CRISPR/Cas and other targeted endonuclease technologies in many species has led to an explosion in the generation of new mutations and alleles. The ability to generate many different mutations from the same target sequence either by homology-directed repair with a donor sequence or non-homologous end joining-induced insertions and deletions necessitates a means for representing these mutations in literature and databases. Standardized nomenclature can be used to generate unambiguous, concise, and specific symbols to represent mutations and alleles.

Zebrafish Mir antagonizes Frizzled 7-induced gastrulation defects.

Although Xenopus studies have shown that Frizzled 7 activates at least two noncanonical signaling pathways, it is less clear which, if any, of these signaling pathways are downstream of Frizzled 7 in zebrafish. Cotransfection of Frizzled 7a (Fz7a) or Frizzled 7 (Fz7) and Xenopus Dsh-GFP results in the translocation of Dsh from the cytoplasm to the plasma membrane, a key step in canonical and noncanonical Wnt signaling. Overexpression of both zebrafish Frizzled 7 orthologs perturbs gastrulation, leading to tail defects.

A PATO-compliant zebrafish screening database (MODB): management of morpholino knockdown screen information.

Background: The zebrafish is a powerful model vertebrate amenable to high throughput in vivo genetic analyses. Examples include reverse genetic screens using morpholino knockdown, expression-based screening using enhancer trapping and forward genetic screening using transposon insertional mutagenesis. We have created a database to facilitate web-based distribution of data from such genetic studies.

The zebrafish band 4.1 member Mir is involved in cell movements associated with gastrulation.

Cellular processes rely on dynamic events occurring between the cortical cytoskeleton and plasma membrane. Members of the Band 4.1 superfamily, which are best known for their ability to tether the cytoskeleton to the plasma membrane, play prominent structural and regulatory roles that influence cell-cell and cell-substrate interactions, endo- and exocytosis, cell polarity, migration, proliferation, and differentiation.