Publications by authors named "Michelle K Bull"
Article Synopsis
- - Lactic acid bacteria play crucial roles in various industries, and accurately identifying their species from mixed environments is essential but challenging due to genetic similarities among species.
- - Traditional amplicon-based methods, like short-read sequencing of the 16S rRNA gene, often struggle with species-level resolution, which can be improved by using long-read sequencing (LRS) techniques.
- - In experiments comparing different amplicons, LRS using V3-V4 yielded lower accuracy in species identification and higher false positives compared to complete 16S rRNA gene and 16S-ITS-23S rRNA region amplicons, emphasizing the need for careful method selection in microbial community analysis.
Mathematical models were developed to predict time to inactivation (TTI) by high-pressure processing of Salmonella in Australian Valencia orange juice (pH 4.3) and navel orange juice (pH 3.7) as a function of pressure magnitude (300 to 600 MPa) and inoculum level (3 to 7 log CFU/ml).
View Article and Find Full Text PDFA five-isolate cocktail of Listeria monocytogenes (10(3) cfu/ml in skim or whole raw milk) was subjected to 450 MPa for 900 s or 600 MPa for 90 s. The effects of prior growth temperature, type of milk (skim vs. whole), type of recovery-enrichment media (optimized Penn State University [oPSU] broth, Listeria Enrichment Broth [LEB], Buffered LEB [BLEB], Modified BLEB [MBLEB], and milk), storage temperature and storage time on the recovery of L.
View Article and Find Full Text PDF