Publications by authors named "Michelle E Grunberg"
Localization of oskar mRNA to the posterior of the Drosophila oocyte is essential for abdominal patterning and germline development. oskar localization is a multi-step process involving temporally and mechanistically distinct transport modes. Numerous cis-acting elements and trans-acting factors have been identified that mediate earlier motor-dependent transport steps leading to an initial accumulation of oskar at the posterior.
View Article and Find Full Text PDFThe widespread transmission and evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) call for rapid nucleic acid diagnostics that are easy to use outside of centralized clinical laboratories. Here we report the development and performance benchmarking of Cas13-based nucleic acid assays leveraging lyophilised reagents and fast sample inactivation at ambient temperature. The assays, which we named SHINEv.
View Article and Find Full Text PDFArticle Synopsis
- The COVID-19 pandemic highlighted the urgent need for widespread and decentralized nucleic acid testing, especially for SARS-CoV-2 Variants of Concern (VOCs).
- SHINEv2 is a new diagnostic tool that uses Cas13 technology, allowing for quick testing at room temperature with easy-to-use, lyophilized reagents.
- In tests, SHINEv2 showed 50 times more sensitivity and 100% specificity compared to leading antigen tests, and can identify multiple VOCs in under 90 minutes without any special equipment.