Development of a High-Throughput Gene Expression Screen for Modulators of RAS-MAPK Signaling in a Mutant RAS Cellular Context.

The RAS-MAPK pathway controls many cellular programs, including cell proliferation, differentiation, and apoptosis. In colorectal cancers, recurrent mutations in this pathway often lead to increased cell signaling that may contribute to the development of neoplasms, thereby making this pathway attractive for therapeutic intervention. To this end, we developed a 26-member gene signature of RAS-MAPK pathway activity utilizing the Affymetrix QuantiGene Plex 2.

Thermoregulatory, cardiovascular, and perceptual responses to intermittent cooling during exercise in a hot, humid outdoor environment.

Decreasing core body temperature during exercise may improve exercise tolerance, facilitate acclimatization, and prevent heat illness during summer training. We sought to evaluate the effectiveness of intermittent superficial cooling on thermoregulatory, cardiovascular, and perceptual responses during exercise in a hot humid environment. We used a randomized, counterbalanced, repeated measures investigation with 2 conditions (control and cooling) during exercise and recovery outdoors on artificial turf in a hot, humid tropical climate in the sun (wet bulb globe temperature outdoors [WBGTo], 27.

Superficial cooling does not decrease core body temperature before, during, or after exercise in an American football uniform.

The purpose of this study was to identify the effects of superficial cooling on thermoregulatory responses while exercising in a hot humid environment while wearing an American football uniform. Nine male and female subjects wore a superficial cooling garment while in a cooling (CS) experimental condition or a no cooling (NCS) control condition during an exercise task consisting of warm-up (WU), exercise (EX), and recovery (R). The exercise task simulated an American football conditioning session with subjects wearing a full American football uniform and performing anaerobic and aerobic exercises in a hot humid environment.

Hydration behaviors before and after an educational and prescribed hydration intervention in adolescent athletes.

Context: The effectiveness of education in modifying hydration behaviors in adolescent athletes is unclear.

Objective: To assess the hydration status and behaviors of female athletes before and after a 1-time educational intervention and prescribed hydration intervention in a warm, humid, tropical environment.

Design: Cohort study.

Exertional rhabdomyolysis in an adolescent athlete during preseason conditioning: a perfect storm.

Cleary, MA, Sadowski, KA, Lee, SY-C, Miller, GL, and Nichols, AW. Exertional rhabdomyolysis in an adolescent athlete during preseason conditioning: a perfect storm. J Strength Cond Res 25(12): 3506-3513, 2011-The purpose of this brief review is to present a case of a healthy, male adolescent athlete (age = 16 years, body mass = 67.

Comparisons of age-predicted maximum heart rate equations in college-aged subjects.

This study investigated the accuracy of age-predicted equations to predict heart rate maximum (HRmax) in a college-age sample and establish efficacy of short-duration anaerobic capacity tests to determine the actual HRmax. A criterion HRmax (CHRmax) was obtained from 96 (52 men and 44 women, age = 22.0 ± 2.

Development of a heat-illness screening instrument using the Delphi panel technique.

Context: Exertional heat illness (EHI) is the third leading cause of death among athletes, but with preparticipation screening, risk factors can be identified, and some EHIs can be prevented.

Objective: To establish content validity of the Heat Illness Index Score (HIIS), a 10-item screening instrument designed to identify athletes at risk for EHI during a preparticipation examination.

Design: Delphi study.

Active dehydration impairs upper and lower body anaerobic muscular power.

We examined the effects of active dehydration by exercise in a hot, humid environment on anaerobic muscular power using a test-retest (euhydrated and dehydrated) design. Seven subjects (age, 27.1 +/- 4.

Thermoregulatory influence of a cooling vest on hyperthermic athletes.

Context: Athletic trainers must have sound evidence for the best practices in treating and preventing heat-related emergencies and potentially catastrophic events.

Objective: To examine the effectiveness of a superficial cooling vest on core body temperature (T(c)) and skin temperature (T(sk)) in hypohydrated hyperthermic male participants.

Design: A randomized control design with 2 experimental groups.

Dehydration and symptoms of delayed-onset muscle soreness in normothermic men.

Context: A dehydrated individual who performs eccentric exercise may exacerbate skeletal muscle damage, leading to structural, contractile, and enzymatic protein denaturation, in addition to the myofiber and connective damage resulting from the eccentric muscle tension.

Objective: To identify the effects of dehydration on 5 physiologic characteristics of delayed-onset muscle soreness (DOMS) in normothermic men after an eccentric exercise perturbation.

Design: Randomized group test-retest design.

Celiac disease in an elite female collegiate volleyball athlete: a case report.

Objective: To present the case of an elite female volleyball player who complained of diarrhea and fatigue after preseason training.

Background: The athlete lost 8.1 kg during the first 20 days of training, and we initially suspected an eating disorder.

Dehydration and symptoms of delayed-onset muscle soreness in hyperthermic males.

Context: Exercise in the heat produces cellular conditions that may leave skeletal muscle susceptible to exercise-induced microdamage. Delayed-onset muscle soreness (DOMS) is a clinical model of contraction-induced skeletal muscle injury.

Objective: To determine whether thermoregulation during exercise heat stress adversely affects muscle injury and the accompanying DOMS.

Temporal Pattern of the Repeated Bout Effect of Eccentric Exercise on Delayed-Onset Muscle Soreness.

OBJECTIVE: To determine the temporal pattern of the repeated bout effect of eccentric exercise on perceived pain and muscular tenderness associated with delayed-onset muscle soreness (DOMS). DESIGN AND SETTING: Subjects completed 2 identical eccentric exercise bouts separated by 6, 7, 8, or 9 weeks. The experiment was conducted in a biokinetics research laboratory.