Publications by authors named "Michela Maresca"

Gene expression is orchestrated by transcription factors, which function within the context of a three-dimensional genome. Zinc-finger protein 143 (ZNF143/ZFP143) is a transcription factor that has been implicated in both gene activation and chromatin looping. To study the direct consequences of ZNF143/ZFP143 loss, we generated a ZNF143/ZFP143 depletion system in mouse embryonic stem cells.

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Genome-wide transcriptional activity involves the binding of many transcription factors (TFs) to thousands of sites in the genome. Pioneer TFs are a class of TFs that maintain open chromatin and allow non-pioneer TFs access to their target sites. Determining which TF binding sites directly drive transcription remains a challenge.

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Article Synopsis
  • The organization of the genome in the nucleus is crucial for various nuclear processes, requiring effective strategies to study gene function.
  • Recent advancements in acute depletion methods, like auxin inducible degron and dTAG, allow researchers to rapidly remove proteins from cells for analysis.
  • This text discusses best practices for creating and validating knockin cell lines to enhance the understanding of 3D genome organization and its regulatory mechanisms.
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The cohesin complex has an essential role in maintaining genome organization. However, its role in gene regulation remains largely unresolved. Here we report that the cohesin release factor WAPL creates a pool of free cohesin, in a process known as cohesin turnover, which reloads it to cell-type-specific binding sites.

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