Transcriptional and biochemical regulation of a novel Arabidopsis thaliana bifunctional aspartate kinase-homoserine dehydrogenase gene isolated by functional complementation of a yeast hom6 mutant.

An aspartate kinase-homoserine dehydrogenase (AK-HSDH) cDNA of Arabidopsis thaliana has been cloned by functional complementation of a Saccharomyces cerevisiae strain mutated in its homoserine dehydrogenase (HSDH) gene (hom6). Two of the three isolated clones were also able to complement a mutant yeast aspartate kinase (AK) gene (hom3). Sequence analysis showed that the identified gene (akthr2), located on chromosome 4, is different from the previously cloned A.

Sequence analysis, transcriptional and posttranscriptional regulation of the rice vdac family.

The voltage-dependent anion-selective channel (VDAC) is a mitochondrial outer membrane ion channel. Different isoforms exist in plants but information about their specific role remains to be established. Our purpose is to find out the structural features common to three rice VDAC isoforms and to investigate their (post)transcriptional regulation in response to an osmotic stress.

Expression of the rice vdac isoform2: histochemical localization and expression level.

The voltage-dependent anion-selective channel (VDAC) is a mitochondrial outer membrane ion channel. The putative promoter of the rice vdac isoform2 (osvdac2) was isolated by screening a rice genomic library. Computer-based analyses predicted a TATA box, a putative transcription start and several transcription factor-binding sites including pollen specific elements.