Usefulness and influence of age of a novel Rapid HpStAR stool antigen for the diagnosis of Helicobacter pylori infection in children.

A novel rapid monoclonal enzyme immunoassay stool antigen for Helicobacter pylori detection (Rapid HpStAR) was evaluated in 16 infected and 92 noninfected children. The overall sensitivity, specificity, and positive and negative predictive values were 87.5%, 97.

Using macro-arrays to study routes of infection of Helicobacter pylori in three families.

Background: Analysis of the evolutionary dynamics of Helicobacter pylori allowed tracing the spread of infection through populations on different continents but transmission pathways between individual humans have not been clearly described.

Materials And Methods: To investigate person-to-person transmission, we studied three families each including one child with persistence of symptoms after antibiotic treatment. Ten isolates from the antrum and corpus of stomach of each family member were analyzed both by sequencing of two housekeeping genes and macroarray tests.

[Helicobacter pylori infection in children].

Knowledge about Helicobacter pylori infection in children continues to advance. While its prevalence appears to be falling in developed countries, it remains a major problem in developing nations. Its transmission pathway remains highly controversial.

Helicobacter pylori primary resistant strains over 11 years in French children.

The yearly prevalence between 1994 and 2005 of primary resistance to amoxicillin, metronidazole, and clarithromycin of 377 Helicobacter pylori strains isolated from children was studied. All the H. pylori strains were susceptible to amoxicillin, 138/377 (36.

Clarithromycin resistance in Helicobacter pylori strains isolated from French children: prevalence of the different mutations and coexistence of clones harboring two different mutations in the same biopsy.

Background: The aim of our study was to assess the different mutations involved in clarithromycin-resistant Helicobacter pylori strains isolated from French children and their temporal trends.

Methods: The point mutations of H. pylori were detected by PCR followed by RFLP technique in 50 clarithromycin-resistant strains collected between 1993 and 2004 in France.

Heterogeneous susceptibility to metronidazole and clarithromycin of Helicobacter pylori isolates from a single biopsy in adults is confirmed in children.

The aim of this study was to assess the resistance to metronidazole and clarithromycin of individual colonies of Helicobacter pylori from a single biopsy taken from 14 adults and 14 children. The Etest was used to determine the minimum inhibitory concentrations (MICs) of these two antimicrobial drugs for ten individual H. pylori colonies isolated from each initial gastric biopsy culture.

Usefulness and influence of age of a novel rapid monoclonal enzyme immunoassay stool antigen for the diagnosis of Helicobacter pylori infection in children.

A novel rapid monoclonal enzyme immunoassay stool antigen for Helicobacter pylori detection (Immunocard STAT!HpSA, Meridian Diagnostic Inc , Cincinnati, OH) was evaluated in 29 infected and 99 noninfected children. The overall sensitivity, specificity, and positive and negative predictive values were 86.2%, 92.

Genetic and transmission analysis of Helicobacter pylori strains within a family.

To look for evidence of intrafamilial infection, we isolated 107 Helicobacter pylori clones from biopsied specimens taken from both parents and four children. We compared the sequences of two housekeeping genes (hspA and glmM) from these clones with those of 131 unrelated strains from patients living in different geographic regions. Strain relationships within the family were determined by analyzing allelic variation at both loci and building phylogenetic trees and by using multilocus sequence typing.