New Detector Sensitivity Calibration and the Calculation of the Interaction Force between Particles Using an Optical Tweezer.

We propose a new approach to calculate the sensitivity factor of the detector in optical tweezers. In this work, we used a charge-coupled device (CCD) camera and a quadrant photodiode (QPD) for the extraction of the various positions occupied by the trapped object (in this case, silica beads of different diameters). Image-J software and the Boltzmann statistical method were then used to estimate the sensitivity of the detector.