Development of a PCR-based assay for rapid detection of class IIa bacteriocin genes.

Aims: We have developed a PCR-based assay using custom designed panel of primers which allows rapid detection of class IIa bacteriocin-coding genes. To demonstrate the applicability of the developed assay, the method was applied on 40 metagenomic DNA preparations isolated from native microbiota of Polish artisanal cheeses produced in the Tatra Mountains.

Methods And Results: The developed assay was designed on the basis of a large scale alignment of class IIa bacteriocin-coding genes.